BMRBj - BMREntryMaster

Found 1 Document (0.321 seconds)

BMRB: 18872

2M1O

ID3 stem

Authors

Popovic, M., Greenbaum, N.L.

Assembly

ID3 stem

Entity

1. RNA GGGUGUA (polymer, Thiol state: not present), 7 monomers, 2340.375 Da Detail

GGGUGUA

2. RNA AGCACCC (polymer, Thiol state: not present), 7 monomers, 2242.359 Da Detail

AGCACCC

Total weight

4582.734 Da

Max. entity weight

2340.375 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 58.6 %, Completeness (bb): 52.6 % Detail

Polymer type: polyribonucleotide

	Total	¹H	¹³C
All	58.6 % (126 of 215)	54.9 % (67 of 122)	63.4 % (59 of 93)
Suger, PO4	52.6 % (81 of 154)	52.4 % (44 of 84)	52.9 % (37 of 70)
Nucleobase	73.8 % (45 of 61)	60.5 % (23 of 38)	95.7 % (22 of 23)
Aromatic	84.9 % (45 of 53)	76.7 % (23 of 30)	95.7 % (22 of 23)

1. RNA GGGUGUA

GGGUGUA

2. RNA AGCACCC

AGCACCC

Show sample condition

Sample

Solvent system 100% D2O, Pressure 1 atm, Temperature 288 K, pH 6.4

#	Name	Isotope labeling	Concentration
1	RNA_GGGUGUA	natural abundance	1 mM
2	RNA_AGCACCC	natural abundance	1 mM
3	sodium chloride	natural abundance	80 mM
4	EDTA	natural abundance	0.1 mM

Release date

2012-12-12

Citation

ID3 stem
Popovic, M., Greenbaum, N.
Not known

Experiments performed 4 experiments Detail

NMR combined restraints 3 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints	combined_18872_2m1o.nef
Input source #2: Coordindates	2m1o.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

-------
GGGUGUA
|||||||
GGGUGUA

Entity_assembly_ID (NMR): B vs Auth_asym_ID (model): B

--20---
AGCACCC
|||||||
AGCACCC
-------

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	7	0	0	100.0
B	B	7	0	0	100.0

Content subtype: combined_18872_2m1o.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	OK	132		100.0 (chain: A, length: 7), 100.0 (chain: B, length: 7)
1	Distance restraints	CNS/XPLOR_distance_constraints_2	OK	94 (1)	noe	100.0 (chain: A, length: 7), 100.0 (chain: B, length: 7)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 7, Sequence coverage: 100.0%
  - Entity_assembly_ID: B, Chain length: 7, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 73
  - ¹³C chemical shifts: 59
- Completeness of assignments
  - Entity_assemble_ID: A
  - Entity_assemble_ID: B
- Histogram of normalized assigned chemical shifts

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	58	37	63.8
¹³C chemical shifts	45	30	66.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	42	27	64.3
¹³C chemical shifts	35	20	57.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	16	10	62.5
¹³C chemical shifts	10	10	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	6	6	100.0
¹³C chemical shifts	6	6	100.0

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
17	A	HO2'	4.964
19	C	HO2'	4.367
20	A	HO2'	4.583
21	C	HO2'	4.24
22	C	HO2'	4.287
23	C	HO2'	4.047

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	64	30	46.9
¹³C chemical shifts	48	29	60.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	42	17	40.5
¹³C chemical shifts	35	17	48.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	22	13	59.1
¹³C chemical shifts	13	12	92.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	5	5	100.0
¹³C chemical shifts	5	5	100.0

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_2
- Status: OK
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 7, Sequence coverage: 100.0%
  - Entity_assembly_ID: B, Chain length: 7, Sequence coverage: 100.0%
- Total number of restraints: 94
- Weight of restraints: 1.0 (94)
- Potential type of restraints: square-well-parabolic (94)
- Range of distance target values: 2.65, 5.11 (Å)
- Histogram of distance target values
- Distance restraints per residue
  - Chain_ID: A
  - Chain_ID: B
- Distance restraints on contact map

Keywords ai5 gamma, group II intron, ID3, stem

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Found 1 Document (0.321 seconds)

BMRB: 18872

Sample

Experiments performed 4 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

NMR combined restraints 3 contents Detail