BMRB: 18878

Solution structure of the Hs. PSIP1 PWWP domain

Authors

van Ingen, H., Van nuland, R., Timmers, H.T.M., Boelens, R.

Assembly

Solution structure of the Hs. PSIP1 PWWP domain

Entity

1. Solution structure of the Hs. PSIP1 PWWP domain (polymer, Thiol state: not present), 105 monomers, 11883.43 Da Detail

GSHMAMARDF KPGDLIFAKM KGYPHWPARV DEVPDGAVKP PTNKLPIFFF GTHETAFLGP KDIFPYSENK EKYGKPNKRK GFNEGLWEID NNPKVKFSSQ QAATK

Formula weight

11883.43 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

CYANA AND WATER-REFINEMENT IN CNS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.4 %, Completeness: 86.0 %, Completeness (bb): 88.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	86.0 % (1102 of 1282)	87.5 % (594 of 679)	83.2 % (415 of 499)	89.4 % (93 of 104)
Backbone	88.7 % (539 of 608)	89.4 % (186 of 208)	87.6 % (268 of 306)	90.4 % (85 of 94)
Sidechain	84.3 % (649 of 770)	86.6 % (408 of 471)	80.6 % (233 of 289)	80.0 % (8 of 10)
Aromatic	66.0 % (99 of 150)	82.7 % (62 of 75)	47.9 % (35 of 73)	100.0 % (2 of 2)
Methyl	88.9 % (64 of 72)	88.9 % (32 of 36)	88.9 % (32 of 36)

1. PC4 AND SFRS1-INTERACTING PROTEIN

GSHMAMARDF KPGDLIFAKM KGYPHWPARV DEVPDGAVKP PTNKLPIFFF GTHETAFLGP KDIFPYSENK EKYGKPNKRK GFNEGLWEID NNPKVKFSSQ QAATK

Show sample condition

Sample

Solvent system 90% water 10% D2O 20 mM NaPi pH 6 100 mM NaCl, Pressure 1.000 atm, Temperature 298.000 K, pH 6.000, Details 0.5 mM

#	Name	Isotope labeling	Type	Concentration
1	PC4 AND SFRS1-INTERACTING PROTEIN	[U-13C; U-15N]		0.5 mM
2	NaPi	natural abundance		20 mM
3	NaCl	natural abundance		100 mM

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LACS Plot; CA

Referencing offset: 0.04 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	HDO	water protons	4.7 ppm	internal	indirect	0.2514495
1H	HDO	water protons	4.7 ppm	internal	indirect	1.0
15N	HDO	water protons	4.7 ppm	internal	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.04 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	HDO	water protons	4.7 ppm	internal	indirect	0.2514495
1H	HDO	water protons	4.7 ppm	internal	indirect	1.0
15N	HDO	water protons	4.7 ppm	internal	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	HDO	water protons	4.7 ppm	internal	indirect	0.2514495
1H	HDO	water protons	4.7 ppm	internal	indirect	1.0
15N	HDO	water protons	4.7 ppm	internal	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.11 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	HDO	water protons	4.7 ppm	internal	indirect	0.2514495
1H	HDO	water protons	4.7 ppm	internal	indirect	1.0
15N	HDO	water protons	4.7 ppm	internal	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 3ZEH, Strand ID: A Detail

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Release date

2013-06-03

Citation

Nucleosomal DNA binding drives the recognition of H3K36-methylated nucleosomes by the PSIP1-PWWP domain
van Nuland, R., van Schaik, F., Simonis, M., van Heesch, S., Cuppen, E., Boelens, R., Timmers, H., van Ingen, H.
Epigenetics. Chromatin (2013), 6, 12-12, PubMed 23656834 , DOI 10.1186/1756-8935-6-12 , Abstract

Related entities 1. Solution structure of the Hs. PSIP1 PWWP domain, : 1 : 4 : 71 entities Detail

Interaction partners 1. Solution structure of the Hs. PSIP1 PWWP domain, : 27 interactors Detail

Experiments performed 2 experiments Detail