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BMRB: 18883

2M1X

TICAM-1 TIR domain structure

Authors

Enokizono, Y., Kumeta, H., Funami, K., Horiuchi, M., Sarmiento, J., Yamashita, K., Standley, D.M., Matsumoto, M., Seya, T., Inagaki, F.

Assembly

TICAM-1 TIR domain

Entity

1. TICAM-1 TIR domain (polymer), 168 monomers, 19102.43 Da Detail

MESSSEQKFY NFVILHARAD EHIALRVREK LEALGVPDGA TFCEDFQVHG RGELSCLQDA IDHSAFIILL LTSNFDCRLS LHQVNQAMMS NLTRQGSPDC VIPFLPLESS PAQLSSDTAS LLSGLVRLDE HSQIFARKVA NTFKPHRLQA RKAMWRKEQD LEHHHHHH

Formula weight

19102.43 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.3 %, Completeness (bb): 99.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.3 % (1907 of 1960)	98.3 % (1001 of 1018)	95.7 % (731 of 764)	98.3 % (175 of 178)
Backbone	99.3 % (989 of 996)	99.4 % (334 of 336)	99.4 % (495 of 498)	98.8 % (160 of 162)
Sidechain	95.9 % (1080 of 1126)	97.8 % (667 of 682)	93.0 % (398 of 428)	93.8 % (15 of 16)
Aromatic	73.5 % (119 of 162)	84.0 % (68 of 81)	62.5 % (50 of 80)	100.0 % (1 of 1)
Methyl	100.0 % (182 of 182)	100.0 % (91 of 91)	100.0 % (91 of 91)

1. TICAM1TIR

MESSSEQKFY NFVILHARAD EHIALRVREK LEALGVPDGA TFCEDFQVHG RGELSCLQDA IDHSAFIILL LTSNFDCRLS LHQVNQAMMS NLTRQGSPDC VIPFLPLESS PAQLSSDTAS LLSGLVRLDE HSQIFARKVA NTFKPHRLQA RKAMWRKEQD LEHHHHHH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.0, Details TICAM-1 TIR (387-545)

#	Name	Isotope labeling	Concentration
1	TICAM-1 TIR	[U-99% 13C; U-99% 15N]	0.5 mM
2	AcOH Buffer	natural abundance	20 mM
3	DTT	natural abundance	5 mM
4	DSS	natural abundance	0.02 mg/mL
5	H20	natural abundance	90 %
6	D20	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.21 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2M1X, Strand ID: A Detail

Release date

2012-12-20

Citation

Structures and interface mapping of the TIR domain-containing adaptor molecules involved in interferon signaling
Enokizono, Y., Kumeta, H., Funami, K., Horiuchi, M., Sarmiento, J., Yamashita, K., Standley, D.M., Matsumoto, M., Seya, T., Inagaki, F.
Proc. Natl. Acad. Sci. U. S. A. (2013), 110, 19908-19913, PubMed 24255114 , DOI 10.1073/pnas.1222811110 , Abstract

Related entities 1. TICAM-1 TIR domain, : 1 : 13 entities Detail

Interaction partners 1. TICAM-1 TIR domain, : 13 interactors Detail

More details for 13 interactors identified by 8 citations

Experiments performed 17 experiments Detail

1 2D 1H-15N HSQC