Solution structure of the Tetrahymena telomerase RNA stem IV terminal loop
Polymer type: polyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 95.9 % (325 of 339) | 96.2 % (179 of 186) | 98.6 % (141 of 143) | 50.0 % (5 of 10) |
Suger, PO4 | 100.0 % (231 of 231) | 100.0 % (126 of 126) | 100.0 % (105 of 105) | |
Nucleobase | 87.0 % (94 of 108) | 88.3 % (53 of 60) | 94.7 % (36 of 38) | 50.0 % (5 of 10) |
Aromatic | 89.6 % (86 of 96) | 93.8 % (45 of 48) | 94.7 % (36 of 38) | 50.0 % (5 of 10) |
1. stemIV telo RNA
GGCGAUACAC UAUUUAUCGC CSolvent system 90% H2O/10% D2O or 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.3
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | stemIV_telo_RNA | unlabeled | 0.6 ~ 1.0 mM | |
2 | potassium chloride | natural abundance | 20 mM | |
3 | sodium azide | natural abundance | 0.2 % | |
4 | EDTA | natural abundance | 50 uM | |
5 | sodium phosphate | natural abundance | 10 mM | |
6 | H2O | natural abundance | 90 % | |
7 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O or 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.3
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | stemIV_telo_RNA | 13C,15N uniformly labelled | 0.6 ~ 1.0 mM | |
9 | sodium phosphate | natural abundance | 10 mM | |
10 | potassium chloride | natural abundance | 20 mM | |
11 | sodium azide | natural abundance | 0.2 % | |
12 | EDTA | natural abundance | 50 uM | |
13 | H2O | natural abundance | 90 % | |
14 | D2O | natural abundance | 10 % |