BMRBj - BMREntryMaster

Found 1 Document (0.806 seconds)

BMRB: 18919

2M33

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Full-Length Rabbit Cytochrome b5

Authors

Subramanian, V., Ahuja, S., Popovych, N., Huang, R., Le Clair, S.V., Jahr, N., Soong, R., Xu, J., Yamamoto, K., Nanga, R.P., Im, S., Waskell, L., Ramamoorthy, A.

Assembly

Full-length cytochrome b5 with heme B

Entity

1. Full-length cytochrome b5 (polymer, Thiol state: not present), 134 monomers, 15349.05 Da Detail

MAAQSDKDVK YYTLEEIKKH NHSKSTWLIL HHKVYDLTKF LEEHPGGEEV LREQAGGDAT ENFEDVGHST DARELSKTFI IGELHPDDRS KLSKPMETLI TTVDSNSSWW TNWVIPAISA LIVALMYRLY MADD

2. Full-length cytochrome b5 with heme B, entity HEB (non-polymer), 618.503 Da

Total weight

15967.553 Da

Max. entity weight

15349.05 Da

Entity Connection

metal coordination 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	metal coordination	sing	1:HIS44:HE2	2:HEB1:FE
2	metal coordination	sing	1:HIS68:HE2	2:HEB1:FE

Source organism

Oryctolagus cuniculus

Exptl. method

solution NMR

Refine. method

distance geometry, torsion angle dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.8 %, Completeness: 60.4 %, Completeness (bb): 60.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	60.4 % (947 of 1568)	76.7 % (620 of 808)	35.6 % (221 of 620)	75.7 % (106 of 140)
Backbone	60.8 % (484 of 796)	83.7 % (226 of 270)	40.2 % (159 of 396)	76.2 % (99 of 130)
Sidechain	58.2 % (524 of 900)	73.4 % (395 of 538)	34.7 % (122 of 352)	70.0 % (7 of 10)
Aromatic	29.5 % (43 of 146)	50.7 % (37 of 73)	7.2 % (5 of 69)	25.0 % (1 of 4)
Methyl	56.7 % (85 of 150)	73.3 % (55 of 75)	40.0 % (30 of 75)

1. Full-length cytochrome b5

MAAQSDKDVK YYTLEEIKKH NHSKSTWLIL HHKVYDLTKF LEEHPGGEEV LREQAGGDAT ENFEDVGHST DARELSKTFI IGELHPDDRS KLSKPMETLI TTVDSNSSWW TNWVIPAISA LIVALMYRLY MADD

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 100 mM potassium phosphate, 5% glycerol, pH 7.4 45 mM DPC micelles

#	Name	Isotope labeling	Concentration
1	Full-length cytochrome b5	[U-100% 13C; U-100% 15N; U-80% 2H]	0.1 ~ 0.5 mM
2	Heme B	natural abundance	0.1 ~ 0.5 mM
3	potassium phosphate	natural abundance	100 mM
4	glycerol	natural abundance	5 %
5	DPC micelles	natural abundance	45 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.22 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.22 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.23 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 23 models in PDB: 2M33, Strand ID: A Detail

Release date

2013-02-04

Citation

¹H, ¹³C and ¹⁵N resonance assignments for the full-length mammalian cytochrome b₅ in a membrane environment
Vivekanandan, S., Ahuja, S., Im, S., Waskell, L., Ramamoorthy, A.
Biomol. NMR Assign. (2014), 8, 409-413, PubMed 24105099 , DOI 10.1007/s12104-013-9528-9 , Abstract

Related entities 1. Full-length cytochrome b5, : 1 : 1 : 46 entities Detail

Interaction partners 1. Full-length cytochrome b5, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC