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BMRB: 18943


18943
3ZFJ
N-terminal domain of pneumococcal PhtD protein with bound Zn(II)
Authors
Bersch, B., Bougault, C., Favier, A., Gabel, F., Roux, L., Vernet, T., Durmort, C.
Assembly
N-terminal domain of pneumococcal PhtD protein with bound Zn(II)
Entity
1. PNEUMOCOCCAL HISTIDINE TRIAD PROTEIN D (polymer, Thiol state: not present), 138 monomers, 15756.20 Da Detail

GGQVKKESNR VSYIDGDQAG QKAENLTPDE VSKREGINAE QIVIKITDQG YVTSHGDHYH YYNGKVPYDA IISEELLMKD PNYQLKDSDI VNEIKGGYVI KVDGKYYVYL KDAAHADNIR TKEEIKRQKQ ERSHNHNS


2. N-terminal domain of pneumococcal PhtD protein with bound Zn(II), entity ZN (non-polymer), 65.409 Da
Total weight
15821.609 Da
Max. entity weight
15756.2 Da
Source organism
Streptococcus pneumoniae
Exptl. method
solution NMR
Refine. method
UNIO10, ARIA
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 91.3 %, Completeness: 85.8 %, Completeness (bb): 89.9 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All85.8 % (1389 of 1618)83.9 % (713 of 850)89.1 % (549 of 616)83.6 % (127 of 152)
Backbone89.9 % (739 of 822)89.4 % (254 of 284)90.1 % (363 of 403)90.4 % (122 of 135)
Sidechain82.9 % (765 of 923)81.1 % (459 of 566)88.5 % (301 of 340)29.4 % (5 of 17)
Aromatic85.7 % (96 of 112)89.3 % (50 of 56)82.1 % (46 of 56)
Methyl93.8 % (122 of 130)93.8 % (61 of 65)93.8 % (61 of 65)

1. PNEUMOCOCCAL HISTIDINE TRIAD PROTEIN D

GGQVKKESNR VSYIDGDQAG QKAENLTPDE VSKREGINAE QIVIKITDQG YVTSHGDHYH YYNGKVPYDA IISEELLMKD PNYQLKDSDI VNEIKGGYVI KVDGKYYVYL KDAAHADNIR TKEEIKRQKQ ERSHNHNS

Show sample condition
Sample #1

Solvent system 90% water, 10% D2O, Pressure 1 atm, Temperature 308.000 K, pH 6.300, Details U15N-U13C-t-PhtD 0.8 mmol/l


#NameIsotope labelingTypeConcentration
1PNEUMOCOCCAL HISTIDINE TRIAD PROTEIN D[U-13C; U-15N]0.8 mM
Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 308.000 K, pH 6.300, Details t-PhtD 0.8 mmol/l


#NameIsotope labelingTypeConcentration
2PNEUMOCOCCAL HISTIDINE TRIAD PROTEIN Dnatural abundance0.8 mM

LACS Plot; CA
Referencing offset: -0.06 ppm, Outliers: 5 Detail
LACS Plot; CB
Referencing offset: -0.06 ppm, Outliers: 5 Detail
LACS Plot; HA
Referencing offset: 0.03 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.09 ppm, Outliers: 3 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 3ZFJ, Strand ID: A Detail

Release date
2013-11-17
Citation 1
New insights into histidine triad proteins: solution structure of a Streptococcus pneumoniae PhtD domain and zinc transfer to AdcAII
Bersch, B., Bougault, C., Roux, L., Favier, A., Vernet, T., Durmort, C.
PLoS One (2013), 8, e81168-e81168, PubMed 24312273 , DOI 10.1371/journal.pone.0081168 ,
Show more 1 citaion
Citation 2
Biochemical characterization of the histidine triad protein PhtD as a cell surface zinc-binding protein of pneumococcus
Loisel, E., Chimalapati, S., Bougault, C., Imberty, A., Gallet, B., Di guilmi, A.M., Brown, J., Vernet, T., Durmort, C.
Biochemistry (2011), 50, 3551-3558, PubMed 21425866 , DOI 10.1021/bi200012f ,
Related entities 1. PNEUMOCOCCAL HISTIDINE TRIAD PROTEIN D, : 1 entities Detail
More details for 1 related entities
Experiments performed 5 experiments Detail
nullKeywords histidine triad, streptococcus, surface protein, ZINC-BINDING PROTEIN, zinc transfer