BMRB: 18950

THE SOLUTION STRUCTURE OF 3',5'-LINKED 2'-O-(2-METHOXYETHYL)-RNA DUPLEX

Authors

Plevnik, M., Cevec, M., Plavec, J.

Assembly

3',5'-LINKED 2'-O-(2-METHOXYETHYL)-RNA DUPLEX

Entity

1. 3',5'-LINKED 2'-O-(2-METHOXYETHYL)-RNA DUPLEX (polymer, Thiol state: not present), 12 monomers, 4597.335 × 2 Da Detail

XXXXXXXXXX XG

Total weight

9194.67 Da

Max. entity weight

4597.335 Da

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chemcal Shifts

¹H: 173 assignments Detail

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ID	Entity ID	Seq ID	Comp ID	Atom ID	Atom type	Val	Val err	Ambig. code
1	1	1	C5L	H1'	H	5.767	0.02	1
2	1	1	C5L	H2'	H	4.443	0.02	1
3	1	1	C5L	H3'	H	4.634	0.02	1
4	1	1	C5L	H41	H	8.403	0.02	1
5	1	1	C5L	H42	H	6.665	0.02	1
6	1	1	C5L	H6	H	7.994	0.02	1
7	1	1	C5L	H7*	H	1.917	0.02	1
8	1	1	C5L	HCA*	H	4.108	0.02	1
9	1	1	C5L	HCD*	H	3.433	0.02	1
10	1	2	G48	H1	H	12.877	0.02	1
11	1	2	G48	H1'	H	6.023	0.02	1
12	1	2	G48	H2'	H	4.463	0.02	1
13	1	2	G48	H3'	H	4.628	0.02	1
14	1	2	G48	H8	H	7.650	0.02	1
15	1	2	G48	HCA*	H	4.349	0.02	1
16	1	2	G48	HCD*	H	3.489	0.02	1
17	1	3	C5L	H1'	H	5.701	0.02	1
18	1	3	C5L	H2'	H	4.477	0.02	1
19	1	3	C5L	H3'	H	4.569	0.02	1
20	1	3	C5L	H41	H	8.786	0.02	1
21	1	3	C5L	H42	H	6.255	0.02	1
22	1	3	C5L	H6	H	7.615	0.02	1
23	1	3	C5L	H7*	H	1.385	0.02	1
24	1	3	C5L	HCA*	H	4.085	0.02	1
25	1	3	C5L	HCD*	H	3.474	0.02	1
26	1	4	G48	H1	H	11.937	0.02	1
27	1	4	G48	H1'	H	5.920	0.02	1
28	1	4	G48	H2'	H	4.477	0.02	1
29	1	4	G48	H3'	H	4.570	0.02	1
30	1	4	G48	H8	H	7.466	0.02	1
31	1	4	G48	HCA*	H	4.278	0.02	1
32	1	4	G48	HCD*	H	3.496	0.02	1
33	1	5	A44	H1'	H	6.069	0.02	1
34	1	5	A44	H2	H	7.202	0.02	1
35	1	5	A44	H2'	H	4.563	0.02	1
36	1	5	A44	H3'	H	4.681	0.02	1
37	1	5	A44	H61	H	7.949	0.02	1
38	1	5	A44	H62	H	6.724	0.02	1
39	1	5	A44	H8	H	7.662	0.02	1
40	1	5	A44	HCA*	H	4.203	0.02	1
41	1	5	A44	HCD*	H	3.472	0.02	1
42	1	6	A44	H1'	H	6.183	0.02	1
43	1	6	A44	H2	H	7.791	0.02	1
44	1	6	A44	H2'	H	4.449	0.02	1
45	1	6	A44	H3'	H	4.327	0.02	1
46	1	6	A44	H61	H	8.382	0.02	1
47	1	6	A44	H62	H	6.752	0.02	1
48	1	6	A44	H8	H	7.386	0.02	1
49	1	6	A44	HCA*	H	4.316	0.02	1
50	1	6	A44	HCD*	H	3.506	0.02	1
51	1	7	T39	H1'	H	5.871	0.02	1
52	1	7	T39	H3	H	13.900	0.02	1
53	1	7	T39	H3'	H	4.400	0.02	1
54	1	7	T39	H6	H	7.310	0.02	1
55	1	7	T39	H7	H	4.225	0.02	1
56	1	7	T39	H7*	H	1.078	0.02	1
57	1	8	T39	H1'	H	5.940	0.02	1
58	1	8	T39	H3	H	13.484	0.02	1
59	1	8	T39	H3'	H	4.505	0.02	1
60	1	8	T39	H6	H	7.849	0.02	1
61	1	8	T39	H7	H	4.252	0.02	1
62	1	8	T39	H7*	H	1.476	0.02	1
63	1	8	T39	HCA*	H	4.188	0.02	1
64	1	9	C5L	H1'	H	5.695	0.02	1
65	1	9	C5L	H2'	H	4.269	0.02	1
66	1	9	C5L	H3'	H	4.581	0.02	1
67	1	9	C5L	H41	H	8.635	0.02	1
68	1	9	C5L	H42	H	6.433	0.02	1
69	1	9	C5L	H6	H	7.793	0.02	1
70	1	9	C5L	H7*	H	1.610	0.02	1
71	1	9	C5L	HCA*	H	4.110	0.02	1
72	1	10	G48	H1	H	12.679	0.02	1
73	1	10	G48	H1'	H	5.831	0.02	1
74	1	10	G48	H2'	H	4.327	0.02	1
75	1	10	G48	H3'	H	4.539	0.02	1
76	1	10	G48	H8	H	7.410	0.02	1
77	1	10	G48	HCA*	H	4.268	0.02	1
78	1	10	G48	HCD*	H	3.473	0.02	1
79	1	11	C5L	H1'	H	5.661	0.02	1
80	1	11	C5L	H2'	H	4.180	0.02	1
81	1	11	C5L	H41	H	8.815	0.02	1
82	1	11	C5L	H42	H	6.266	0.02	1
83	1	11	C5L	H6	H	7.553	0.02	1
84	1	11	C5L	H7*	H	1.317	0.02	1
85	1	11	C5L	HCD*	H	3.503	0.02	1
86	1	12	DG	H1'	H	6.079	0.02	1
87	1	12	DG	H8	H	7.543	0.02	1
88	1	1	C5L	H1'	H	5.767	0.02	1
89	1	1	C5L	H2'	H	4.443	0.02	1
90	1	1	C5L	H3'	H	4.634	0.02	1
91	1	1	C5L	H41	H	8.403	0.02	1
92	1	1	C5L	H42	H	6.665	0.02	1
93	1	1	C5L	H6	H	7.994	0.02	1
94	1	1	C5L	H7*	H	1.917	0.02	1
95	1	1	C5L	HCA*	H	4.108	0.02	1
96	1	1	C5L	HCD*	H	3.433	0.02	1
97	1	2	G48	H1	H	12.877	0.02	1
98	1	2	G48	H1'	H	6.023	0.02	1
99	1	2	G48	H2'	H	4.463	0.02	1
100	1	2	G48	H3'	H	4.628	0.02	1
101	1	2	G48	H8	H	7.650	0.02	1
102	1	2	G48	HCA*	H	4.349	0.02	1
103	1	2	G48	HCD*	H	3.489	0.02	1
104	1	3	C5L	H1'	H	5.701	0.02	1
105	1	3	C5L	H2'	H	4.477	0.02	1
106	1	3	C5L	H3'	H	4.569	0.02	1
107	1	3	C5L	H41	H	8.786	0.02	1
108	1	3	C5L	H42	H	6.255	0.02	1
109	1	3	C5L	H6	H	7.615	0.02	1
110	1	3	C5L	H7*	H	1.385	0.02	1
111	1	3	C5L	HCA*	H	4.085	0.02	1
112	1	3	C5L	HCD*	H	3.474	0.02	1
113	1	4	G48	H1	H	11.937	0.02	1
114	1	4	G48	H1'	H	5.920	0.02	1
115	1	4	G48	H2'	H	4.477	0.02	1
116	1	4	G48	H3'	H	4.570	0.02	1
117	1	4	G48	H8	H	7.466	0.02	1
118	1	4	G48	HCA*	H	4.278	0.02	1
119	1	4	G48	HCD*	H	3.496	0.02	1
120	1	5	A44	H1'	H	6.069	0.02	1
121	1	5	A44	H2	H	7.202	0.02	1
122	1	5	A44	H2'	H	4.563	0.02	1
123	1	5	A44	H3'	H	4.681	0.02	1
124	1	5	A44	H61	H	7.949	0.02	1
125	1	5	A44	H62	H	6.724	0.02	1
126	1	5	A44	H8	H	7.662	0.02	1
127	1	5	A44	HCA*	H	4.203	0.02	1
128	1	5	A44	HCD*	H	3.472	0.02	1
129	1	6	A44	H1'	H	6.183	0.02	1
130	1	6	A44	H2	H	7.791	0.02	1
131	1	6	A44	H2'	H	4.449	0.02	1
132	1	6	A44	H3'	H	4.327	0.02	1
133	1	6	A44	H61	H	8.382	0.02	1
134	1	6	A44	H62	H	6.752	0.02	1
135	1	6	A44	H8	H	7.386	0.02	1
136	1	6	A44	HCA*	H	4.316	0.02	1
137	1	6	A44	HCD*	H	3.506	0.02	1
138	1	7	T39	H1'	H	5.871	0.02	1
139	1	7	T39	H2'	H	4.225	0.02	1
140	1	7	T39	H3	H	13.900	0.02	1
141	1	7	T39	H3'	H	4.400	0.02	1
142	1	7	T39	H6	H	7.310	0.02	1
143	1	7	T39	H7*	H	1.078	0.02	1
144	1	8	T39	H1'	H	5.940	0.02	1
145	1	8	T39	H2'	H	4.252	0.02	1
146	1	8	T39	H3	H	13.484	0.02	1
147	1	8	T39	H3'	H	4.505	0.02	1
148	1	8	T39	H6	H	7.849	0.02	1
149	1	8	T39	H7*	H	1.476	0.02	1
150	1	8	T39	HCA*	H	4.188	0.02	1
151	1	9	C5L	H1'	H	5.695	0.02	1
152	1	9	C5L	H2'	H	4.269	0.02	1
153	1	9	C5L	H3'	H	4.581	0.02	1
154	1	9	C5L	H41	H	8.635	0.02	1
155	1	9	C5L	H42	H	6.433	0.02	1
156	1	9	C5L	H6	H	7.793	0.02	1
157	1	9	C5L	H7*	H	1.610	0.02	1
158	1	9	C5L	HCA*	H	4.110	0.02	1
159	1	10	G48	H1	H	12.679	0.02	1
160	1	10	G48	H1'	H	5.831	0.02	1
161	1	10	G48	H2'	H	4.327	0.02	1
162	1	10	G48	H3'	H	4.539	0.02	1
163	1	10	G48	H8	H	7.410	0.02	1
164	1	10	G48	HCA*	H	4.268	0.02	1
165	1	10	G48	HCD*	H	3.473	0.02	1
166	1	11	C5L	H1'	H	5.661	0.02	1
167	1	11	C5L	H2'	H	4.180	0.02	1
168	1	11	C5L	H41	H	8.815	0.02	1
169	1	11	C5L	H42	H	6.266	0.02	1
170	1	11	C5L	H6	H	7.553	0.02	1
171	1	11	C5L	H7*	H	1.317	0.02	1
172	1	11	C5L	HCD*	H	3.503	0.02	1
173	1	12	DG	H1'	H	6.079	0.02	1

Release date

2013-11-25

Citation

NMR structure of 2'-O-(2-methoxyethyl) modified and C5-methylated RNA dodecamer duplex
Plevnik, M., Cevec, M., Plavec, J.
Biochimie (2013), 95, 2385-2391, PubMed 24012551 , DOI 10.1016/j.biochi.2013.08.025 , Abstract

Experiments performed 3 experiments Detail