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BMRB: 18955

3ZJ1

STRUCTURE OF NAB2P TANDEM ZINC FINGER 12

Authors

Martinez-lubreras, S., Santiveri, C.M., Mirassou, Y., Zorrilla, S., Perez-Canadillas, J.

Assembly

STRUCTURE OF NAB2P TANDEM ZINC FINGER 12

Entity

1. NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2 (polymer, Thiol state: all disulfide bound), 83 monomers, 9575.944 Da Detail

GSFTPTKKEG RCRLFPHCPL GRSCPHAHPT KVCNEYPNCP KPPGTCEFLH PNEDEELMKE MERTREEFQK RKADLLAAKR KPV

2. STRUCTURE OF NAB2P TANDEM ZINC FINGER 12, entity ZN (non-polymer), 65.409 × 2 Da

Total weight

9706.763 Da

Max. entity weight

9575.944 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

Refine. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.6 %, Completeness: 84.5 %, Completeness (bb): 93.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.5 % (838 of 992)	84.1 % (449 of 534)	82.7 % (316 of 382)	96.1 % (73 of 76)
Backbone	93.1 % (443 of 476)	94.3 % (150 of 159)	91.4 % (224 of 245)	95.8 % (69 of 72)
Sidechain	79.0 % (470 of 595)	79.7 % (299 of 375)	77.3 % (167 of 216)	100.0 % (4 of 4)
Aromatic	39.1 % (25 of 64)	78.1 % (25 of 32)	0.0 % (0 of 32)
Methyl	92.0 % (46 of 50)	92.0 % (23 of 25)	92.0 % (23 of 25)

1. NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2

GSFTPTKKEG RCRLFPHCPL GRSCPHAHPT KVCNEYPNCP KPPGTCEFLH PNEDEELMKE MERTREEFQK RKADLLAAKR KPV

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.300 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2	[U-13C; U-15N]		0.300 mM

Sample #2

Solvent system 100% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.300 mmol/l

#	Name	Isotope labeling	Type	Concentration
2	NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2	[U-13C; U-15N]		0.300 mM

LACS Plot; CA

Referencing offset: -0.19 ppm, Outliers: 2 Detail

LACS Plot; CB

Referencing offset: -0.19 ppm, Outliers: 2 Detail

LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 2 Detail

LACS Plot; CO

Referencing offset: -0.26 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 3ZJ1, Strand ID: A Detail

Release date

2013-08-28

Citation

Two singular types of CCCH tandem zinc finger in Nab2p contribute to polyadenosine RNA recognition
Martinez-Lumbreras, S., Santiveri, C.M., Mirassou, Y., Zorrilla, S., Perez-Canadillas, J.
Structure (2013), 21, 1800-1811, PubMed 23994011 , DOI 10.1016/j.str.2013.07.019 , Abstract

Related entities 1. NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2, : 1 : 4 entities Detail

Interaction partners 1. NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2, : 30 interactors Detail

More details for 30 interactors identified by 9 citations

Experiments performed 9 experiments Detail

1 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.300 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2	[U-13C; U-15N]		0.300 mM

2 1H-13C HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.300 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2	[U-13C; U-15N]		0.300 mM

3 HNCO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.300 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2	[U-13C; U-15N]		0.300 mM

4 HNCA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.300 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2	[U-13C; U-15N]		0.300 mM

5 CBCA(CO)HN

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.300 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2	[U-13C; U-15N]		0.300 mM

6 H(C)CH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.300 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2	[U-13C; U-15N]		0.300 mM

7 15N HSQC-NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.300 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2	[U-13C; U-15N]		0.300 mM

8 NOESY and TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.300 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2	[U-13C; U-15N]		0.300 mM

9 NOESY

Instrument

Bruker DMX - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.300 mmol/l

#	Name	Isotope labeling	Type	Concentration
2	NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2	[U-13C; U-15N]		0.300 mM

nullKeywords CCCH-TYPE ZINC FINGER, MRNA EXPORT, POL LENGHT CONTROL, POLYADENOSINE RNA BINDING., POLY(A) LENGHT CONTROL, RNA BINDING PROTEIN