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BMRB: 18956

3ZJ2

STRUCTURE OF NAB2P TANDEM ZINC FINGER 34

Authors

Martinez-lubreras, S., Santiveri, C.M., Mirassou, Y., Zorrilla, S., Perez-Canadillas, J.

Assembly

STRUCTURE OF NAB2P TANDEM ZINC FINGER 34

Entity

1. NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2 (polymer, Thiol state: all disulfide bound), 71 monomers, 7687.826 Da Detail

GSVQTGIVLC KFGALCSNPS CPFGHPTPAN EDAKVIDLMW CDKNLTCDNP ECRKAHSSLS KIKEVKPISQ K

2. STRUCTURE OF NAB2P TANDEM ZINC FINGER 34, entity ZN (non-polymer), 65.409 × 2 Da

Total weight

7818.644 Da

Max. entity weight

7687.826 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

Refine. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.6 %, Completeness (bb): 98.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.6 % (787 of 806)	98.3 % (416 of 423)	97.4 % (303 of 311)	94.4 % (68 of 72)
Backbone	98.8 % (409 of 414)	99.3 % (139 of 140)	99.0 % (207 of 209)	96.9 % (63 of 65)
Sidechain	96.9 % (445 of 459)	97.9 % (277 of 283)	96.4 % (163 of 169)	71.4 % (5 of 7)
Aromatic	85.0 % (34 of 40)	100.0 % (20 of 20)	68.4 % (13 of 19)	100.0 % (1 of 1)
Methyl	100.0 % (66 of 66)	100.0 % (33 of 33)	100.0 % (33 of 33)

1. NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2

GSVQTGIVLC KFGALCSNPS CPFGHPTPAN EDAKVIDLMW CDKNLTCDNP ECRKAHSSLS KIKEVKPISQ K

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.5 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2	[U-13C; U-15N]		0.5 mM

Sample #2

Solvent system 100% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.5 mmol/l

#	Name	Isotope labeling	Type	Concentration
2	NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2	[U-13C; U-15N]		0.5 mM

LACS Plot; CA

Referencing offset: 0.13 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: 0.13 ppm, Outliers: 1 Detail

LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CO

Referencing offset: 0.45 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 3ZJ2, Strand ID: A Detail

Release date

2013-08-28

Citation

Two singular types of CCCH tandem zinc finger in Nab2p contribute to polyadenosine RNA recognition
Martinez-Lumbreras, S., Santiveri, C.M., Mirassou, Y., Zorrilla, S., Perez-Canadillas, J.
Structure (2013), 21, 1800-1811, PubMed 23994011 , DOI 10.1016/j.str.2013.07.019 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 18955 released on 2013-08-28
Title STRUCTURE OF NAB2P TANDEM ZINC FINGER 12

Related entities 1. NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2, : 1 : 2 : 3 entities Detail

Interaction partners 1. NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2, : 30 interactors Detail

More details for 30 interactors identified by 9 citations

Experiments performed 19 experiments Detail

1 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.5 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2	[U-13C; U-15N]		0.5 mM

2 1H-15N HMBC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.5 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2	[U-13C; U-15N]		0.5 mM

3 1H-13C HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.5 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2	[U-13C; U-15N]		0.5 mM

4 HNCO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.5 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2	[U-13C; U-15N]		0.5 mM

5 HN(CA)CO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.5 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2	[U-13C; U-15N]		0.5 mM

6 HNCA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.5 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2	[U-13C; U-15N]		0.5 mM

7 HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.5 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2	[U-13C; U-15N]		0.5 mM

8 CBCA(CO)HN

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.5 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2	[U-13C; U-15N]		0.5 mM

9 H(C)CH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.5 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2	[U-13C; U-15N]		0.5 mM

10 (H)CCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.5 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2	[U-13C; U-15N]		0.5 mM

11 (HB)CB(CGCD)HD Aro

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.5 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2	[U-13C; U-15N]		0.5 mM

12 (HB)CB(CGCDCE)HE Aro

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.5 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2	[U-13C; U-15N]		0.5 mM

13 15N HSQC-NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.5 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2	[U-13C; U-15N]		0.5 mM

14 NOESY and TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.5 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2	[U-13C; U-15N]		0.5 mM

15 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.5 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2	[U-13C; U-15N]		0.5 mM

16 H(C)CH-TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.5 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2	[U-13C; U-15N]		0.5 mM

17 (H)CCH-TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.5 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2	[U-13C; U-15N]		0.5 mM

18 NOESY AND TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.5 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2	[U-13C; U-15N]		0.5 mM

19 NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.5 mmol/l

#	Name	Isotope labeling	Type	Concentration
2	NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2	[U-13C; U-15N]		0.5 mM

nullKeywords CCCH-TYPE ZINC FINGER, MRNA EXPORT, POL LENGTH CONTROL, POLYADENOSINE RNA BINDING, POLYADENOSINE RNA BINDING., POLY(A) LENGHT CONTROL, RNA BINDING PROTEIN