BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	92.6 % (276 of 298)	99.4 % (160 of 161)	82.1 % (92 of 112)	96.0 % (24 of 25)
Backbone	83.3 % (110 of 132)	97.7 % (43 of 44)	69.7 % (46 of 66)	95.5 % (21 of 22)
Sidechain	100.0 % (188 of 188)	100.0 % (117 of 117)	100.0 % (68 of 68)	100.0 % (3 of 3)
Aromatic	100.0 % (24 of 24)	100.0 % (12 of 12)	100.0 % (10 of 10)	100.0 % (2 of 2)
Methyl	100.0 % (18 of 18)	100.0 % (9 of 9)	100.0 % (9 of 9)

1. Rsa1p238-259

TDEDVKKWRE ERKKMWLLKI SN

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 3.7

#	Name	Isotope labeling	Concentration
1	Rsa1p238-259	natural abundance	2 mM
2	potassium phosphate	natural abundance	10 mM
3	sodium chloride	natural abundance	150 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

Chem. Shift Complete2

Sequence coverage: 100.0 %, Completeness: 74.3 %, Completeness (bb): 62.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	74.3 % (443 of 596)	96.9 % (312 of 322)	46.4 % (104 of 224)	54.0 % (27 of 50)
Backbone	62.5 % (165 of 264)	97.7 % (86 of 88)	41.7 % (55 of 132)	54.5 % (24 of 44)
Sidechain	80.6 % (303 of 376)	96.6 % (226 of 234)	54.4 % (74 of 136)	50.0 % (3 of 6)
Aromatic	75.0 % (36 of 48)	100.0 % (24 of 24)	50.0 % (10 of 20)	50.0 % (2 of 4)
Methyl	80.6 % (29 of 36)	100.0 % (18 of 18)	61.1 % (11 of 18)

1. Rsa1p238-259

TDEDVKKWRE ERKKMWLLKI SN

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 305 K, pH 3.7

#	Name	Isotope labeling	Concentration
1	Rsa1p238-259	natural abundance	2 mM
2	potassium phosphate	natural abundance	10 mM
3	sodium chloride	natural abundance	150 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

Protein Blocks Logo

Calculated from 10 models in PDB: 2M3F, Strand ID: A Detail

Release date

2013-11-25

Citation

Characterization of the interaction between protein Snu13p/15.5K and the Rsa1p/NUFIP factor and demonstration of its functional importance for snoRNP assembly
Rothe, B., Back, R., Quinternet, M., Bizarro, J., Robert, M., Blaud, M., Romier, C., Manival, X., Charpentier, B., Bertrand, E., Branlant, C.
Nucleic Acids Res. (2014), 42, 2015-2036, PubMed 24234454 , DOI 10.1093/nar/gkt1091 , Abstract

Related entities 1. Rsa1p238-259, : 1 : 3 entities Detail

Interaction partners 1. Rsa1p238-259, : 8 interactors Detail

More details for 8 interactors identified by 4 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 3.7

#	Name	Isotope labeling	Concentration
1	Rsa1p238-259	natural abundance	2 mM
2	potassium phosphate	natural abundance	10 mM
3	sodium chloride	natural abundance	150 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

2 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 305 K, pH 3.7

#	Name	Isotope labeling	Concentration
1	Rsa1p238-259	natural abundance	2 mM
2	potassium phosphate	natural abundance	10 mM
3	sodium chloride	natural abundance	150 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

3 2D 1H-1H NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 3.7

#	Name	Isotope labeling	Concentration
1	Rsa1p238-259	natural abundance	2 mM
2	potassium phosphate	natural abundance	10 mM
3	sodium chloride	natural abundance	150 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

4 2D 1H-1H NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 305 K, pH 3.7

#	Name	Isotope labeling	Concentration
1	Rsa1p238-259	natural abundance	2 mM
2	potassium phosphate	natural abundance	10 mM
3	sodium chloride	natural abundance	150 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

5 2D 1H-1H TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 3.7

#	Name	Isotope labeling	Concentration
1	Rsa1p238-259	natural abundance	2 mM
2	potassium phosphate	natural abundance	10 mM
3	sodium chloride	natural abundance	150 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

6 2D 1H-1H TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 305 K, pH 3.7

#	Name	Isotope labeling	Concentration
1	Rsa1p238-259	natural abundance	2 mM
2	potassium phosphate	natural abundance	10 mM
3	sodium chloride	natural abundance	150 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

7 2D 1H-13C HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 3.7

#	Name	Isotope labeling	Concentration
1	Rsa1p238-259	natural abundance	2 mM
2	potassium phosphate	natural abundance	10 mM
3	sodium chloride	natural abundance	150 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

8 2D 1H-13C HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 305 K, pH 3.7

#	Name	Isotope labeling	Concentration
1	Rsa1p238-259	natural abundance	2 mM
2	potassium phosphate	natural abundance	10 mM
3	sodium chloride	natural abundance	150 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

9 2D 1H-1H COSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 3.7

#	Name	Isotope labeling	Concentration
1	Rsa1p238-259	natural abundance	2 mM
2	potassium phosphate	natural abundance	10 mM
3	sodium chloride	natural abundance	150 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

10 2D 1H-1H COSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 305 K, pH 3.7

#	Name	Isotope labeling	Concentration
1	Rsa1p238-259	natural abundance	2 mM
2	potassium phosphate	natural abundance	10 mM
3	sodium chloride	natural abundance	150 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

11 2D 1H-13C HSQC-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 3.7

#	Name	Isotope labeling	Concentration
1	Rsa1p238-259	natural abundance	2 mM
2	potassium phosphate	natural abundance	10 mM
3	sodium chloride	natural abundance	150 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

12 2D 1H-13C HSQC-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 305 K, pH 3.7

#	Name	Isotope labeling	Concentration
1	Rsa1p238-259	natural abundance	2 mM
2	potassium phosphate	natural abundance	10 mM
3	sodium chloride	natural abundance	150 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

NMR combined restraints 5 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_18959_2m3f.nef
Input source #2: Coordindates	2m3f.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--
TDEDVKKWREERKKMWLLKISN
||||||||||||||||||||||
TDEDVKKWREERKKMWLLKISN

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	22	0	0	100.0

Content subtype: combined_18959_2m3f.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	276		100.0 (chain: A, length: 22)
2	Assigned chemical shifts	assigned_chem_shift_list_2	OK	152		100.0 (chain: A, length: 22)
1	Distance restraints	CNS/XPLOR_distance_constraints_2	OK	605 (1)	noe	100.0 (chain: A, length: 22)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_3	OK	44 (44)	.	100.0 (chain: A, length: 22)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 22, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 160
  - ¹³C chemical shifts: 90
  - ¹⁵N chemical shifts: 26
- Completeness of assignments
  - Entity_assemble_ID: A
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A
Saveframe: assigned_chem_shift_list_2
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 22, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 152
- Completeness of assignments
  - Entity_assemble_ID: A
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	161	160	99.4
¹³C chemical shifts	112	90	80.4
¹⁵N chemical shifts	27	26	96.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	44	43	97.7
¹³C chemical shifts	44	22	50.0
¹⁵N chemical shifts	22	21	95.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	117	117	100.0
¹³C chemical shifts	68	68	100.0
¹⁵N chemical shifts	5	5	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	10	10	100.0
¹³C chemical shifts	10	10	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	12	12	100.0
¹³C chemical shifts	10	10	100.0
¹⁵N chemical shifts	2	2	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	161	152	94.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	44	43	97.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	117	109	93.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	10	10	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	12	12	100.0

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_2
- Status: OK
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 22, Sequence coverage: 100.0%
- Total number of restraints: 605
- Weight of restraints: 1.0 (605)
- Potential type of restraints: square-well-parabolic (605)
- Range of distance target values: 1.32, 2.75 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_3
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 22, Sequence coverage: 100.0%
- Total number of restraints: 44
- Total number of restraints per polymer type: 44
- Weight of restraints: 1.0 (44)
- Potential type of restraints: square-well-parabolic (44)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A

Keywords helix, peptide

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (0.749 seconds)

BMRB: 18959

Sample

Sample

Related entities 1. Rsa1p238-259, : 1 : 3 entities Detail

Interaction partners 1. Rsa1p238-259, : 8 interactors Detail

Experiments performed 12 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

NMR combined restraints 5 contents Detail