BMRBj - BMREntryMaster

Found 1 Document (1.734 seconds)

BMRB: 18995

2M49

Structural Insights into Human S100B and Basic Fibroblast Growth Factor (FGF2) Interaction

Authors

Gupta, A.A., Yu, C.

Assembly

Human S100B and Basic Fibroblast Growth Factor (FGF2) Interaction

Entity

1. FGF2 (polymer, Thiol state: all free), 126 monomers, 14397.31 × 2 Da Detail

DPKRLYCKNG GFFLRIHPDG RVDGVREKSD PHIKLQLQAE ERGVVSIKGV SANRYLAMKE DGRLLASKSV TDECFFFERL ESNNYNTYRS RKYTSWYVAL KRTGQYKLGS KTGPGQKAIL FLPMSA

2. S100B (polymer, Thiol state: all free), 182 monomers, 21145.43 Da Detail

SELEKAMVAL IDVFHQYSGR EGDKHKLKKS ELKELINNEL SHFLEEIKEQ EVVDKVMETL DNDGDGECDF QEFMAFVAMV TTACHEFFEH ESELEKAMVA LIDVFHQYSG REGDKHKLKK SELKELINNE LSHFLEEIKE QEVVDKVMET LDNDGDGECD FQEFMAFVAM VTTACHEFFE HE

Total weight

49940.047 Da

Max. entity weight

21145.43 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 31.5 %, Completeness: 13.7 %, Completeness (bb): 24.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	13.7 % (502 of 3666)	6.9 % (132 of 1915)	19.6 % (280 of 1426)	27.7 % (90 of 325)
Backbone	24.4 % (449 of 1838)	15.9 % (100 of 630)	28.6 % (259 of 905)	29.7 % (90 of 303)
Sidechain	6.3 % (134 of 2117)	2.6 % (33 of 1285)	12.5 % (101 of 810)	0.0 % (0 of 22)
Aromatic	0.6 % (2 of 332)	0.6 % (1 of 166)	0.6 % (1 of 165)	0.0 % (0 of 1)
Methyl	4.8 % (14 of 292)	3.4 % (5 of 146)	6.2 % (9 of 146)

1. FGF2

DPKRLYCKNG GFFLRIHPDG RVDGVREKSD PHIKLQLQAE ERGVVSIKGV SANRYLAMKE DGRLLASKSV TDECFFFERL ESNNYNTYRS RKYTSWYVAL KRTGQYKLGS KTGPGQKAIL FLPMSA

2. S100B

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Concentration
1	S100B	[U-100% 13C; U-100% 15N]	1.2 mM
2	TRIS	natural abundance	20 mM
3	ammonium sulfate	natural abundance	50 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	5 mM
6	sodium azide	natural abundance	0.01 %
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	na	indirect	0.2514495
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	na	indirect	0.1013291

Referencing offset: -0.23 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	na	indirect	0.2514495
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	na	indirect	0.1013291

Referencing offset: -0.23 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	na	indirect	0.2514495
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	na	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2M49, Strand ID: A, B, C, D Detail

Release date

2013-12-15

Citation

Structural insights into the interaction of human S100B and basic fibroblast growth factor (FGF2): Effects on FGFR1 receptor signaling
Gupta, A.A., Chou, R., Li, H., Yang, L., Yu, C.
Biochim. Biophys. Acta (2013), 1834, 2606-2619, PubMed 24063890 , DOI 10.1016/j.bbapap.2013.09.012 , Abstract

Related entities 1. FGF2, : 3 : 182 entities Detail

Related entities 2. S100B, : 2 entities Detail

Interaction partners 1. FGF2, : 14 interactors Detail

More details for 14 interactors identified by 11 citations

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC