BMRBj - BMREntryMaster

Found 1 Document (0.61 seconds)

BMRB: 19000

2M4E

Solution NMR structure of VV2_0175 from Vibrio vulnificus, NESG target VnR1 and CSGID target IDP91333

Authors

Wu, B., Yee, A., Houliston, S., Lemak, A., Garcia, M., Savchenko, A., Arrowsmith, C.H.

Assembly

VV2 0175

Entity

1. VV2 0175 (polymer, Thiol state: not present), 110 monomers, 12252.01 Da Detail

MHHHHHHSSG VDLGTENLYF QSNAMSKTAK LNNEEKLVKK ALEIGGKMAK MQGFDLPQSP QPVRVKAVYL FLVDAKQIAP LPDSKLDGAN IKHRLALWIH AALPDNDPLK

Formula weight

12252.01 Da

Source organism

Vibrio vulnificus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 77.3 %, Completeness: 73.1 %, Completeness (bb): 69.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	73.1 % (954 of 1305)	75.0 % (512 of 683)	72.4 % (367 of 507)	65.2 % (75 of 115)
Backbone	69.5 % (449 of 646)	69.9 % (153 of 219)	70.1 % (227 of 324)	67.0 % (69 of 103)
Sidechain	76.5 % (584 of 763)	77.4 % (359 of 464)	76.3 % (219 of 287)	50.0 % (6 of 12)
Aromatic	40.0 % (36 of 90)	46.7 % (21 of 45)	31.8 % (14 of 44)	100.0 % (1 of 1)
Methyl	85.2 % (104 of 122)	85.2 % (52 of 61)	85.2 % (52 of 61)

1. VV2 0175

MHHHHHHSSG VDLGTENLYF QSNAMSKTAK LNNEEKLVKK ALEIGGKMAK MQGFDLPQSP QPVRVKAVYL FLVDAKQIAP LPDSKLDGAN IKHRLALWIH AALPDNDPLK

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	VV2_0175	[U-100% 13C; U-100% 15N]	0.5 mM
2	TRIS	[U-100% 2H]	10 mM
3	sodium chloride	natural abundance	300 mM
4	DTT	[U-100% 2H]	10 mM
5	NaN3	natural abundance	0.01 %
6	benzamidine	natural abundance	10 mM
7	inhibitor cocktail	natural abundance	1 na
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
10	VV2_0175	[U-7% 13C; U-100% 15N]	0.2 mM
11	TRIS	[U-100% 2H]	10 mM
12	sodium chloride	natural abundance	300 mM
13	DTT	[U-100% 2H]	10 mM
14	NaN3	natural abundance	0.01 %
15	benzamidine	natural abundance	10 mM
16	inhibitor cocktail	natural abundance	1 na
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.16 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.16 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.16 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.16 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2M4E, Strand ID: A Detail

Release date

2013-02-19

Citation

Solution NMR structure of VV2_0175 from Vibrio vulnificus, NESG target VnR1 and CSGID target IDP91333
Wu, B., Yee, A., Houliston, S., Lemak, A., Garcia, M., Savchenko, A., Arrowsmith, C.H.
Not known

Related entities 1. VV2 0175, : 1 : 4 entities Detail

Experiments performed 16 experiments Detail

1 3D HNCO