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BMRB: 19001

2M4F

OspE

Authors

Bhattacharjee, A., Oeemig, J.S., Kolodziejczyk, R., Meri, T., Kajander, T., Iwai, H., Jokiranta, T., Goldman, A.

Assembly

OspE

Entity

1. OspE (polymer, Thiol state: not present), 151 monomers, 16916.75 Da Detail

KIHTSYDEQS SGESKVKKIE FSKFTVKIKN KDKSGNWTDL GDLVVRKEEN GIDTGLNAGG HSATFFSLEE EVVNNFVKVM TEGGSFKTSL YYGYKEEQSV INGIQNKEII TKIEKIDGTE YITFSGDKIK NSGDKVAEYA ISLEELKKNL K

Formula weight

16916.75 Da

Source organism

Borreliella burgdorferi

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 93.6 %, Completeness (bb): 98.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.6 % (1675 of 1789)	92.3 % (862 of 934)	94.3 % (651 of 690)	98.2 % (162 of 165)
Backbone	98.6 % (893 of 906)	97.2 % (307 of 316)	99.3 % (436 of 439)	99.3 % (150 of 151)
Sidechain	90.0 % (918 of 1020)	89.8 % (555 of 618)	90.5 % (351 of 388)	85.7 % (12 of 14)
Aromatic	68.1 % (94 of 138)	84.1 % (58 of 69)	51.5 % (35 of 68)	100.0 % (1 of 1)
Methyl	99.3 % (151 of 152)	98.7 % (75 of 76)	100.0 % (76 of 76)

1. OspE

KIHTSYDEQS SGESKVKKIE FSKFTVKIKN KDKSGNWTDL GDLVVRKEEN GIDTGLNAGG HSATFFSLEE EVVNNFVKVM TEGGSFKTSL YYGYKEEQSV INGIQNKEII TKIEKIDGTE YITFSGDKIK NSGDKVAEYA ISLEELKKNL K

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Concentration
1	OspE	[U-99% 13C; U-99% 15N]	1 mM
2	sodium phosphate	natural abundance	20 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	0.0 ppm	na	direct	1.0
¹H	water	protons	4.75 ppm	internal	direct	1.0
¹⁵N	liquid anhydrous ammonia	nitrogen	117.8 ppm	na	direct	1.0

Referencing offset: -0.08 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	0.0 ppm	na	direct	1.0
¹H	water	protons	4.75 ppm	internal	direct	1.0
¹⁵N	liquid anhydrous ammonia	nitrogen	117.8 ppm	na	direct	1.0

Referencing offset: -0.08 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	0.0 ppm	na	direct	1.0
¹H	water	protons	4.75 ppm	internal	direct	1.0
¹⁵N	liquid anhydrous ammonia	nitrogen	117.8 ppm	na	direct	1.0

Referencing offset: -0.02 ppm, Outliers: 4 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	0.0 ppm	na	direct	1.0
¹H	water	protons	4.75 ppm	internal	direct	1.0
¹⁵N	liquid anhydrous ammonia	nitrogen	117.8 ppm	na	direct	1.0

Referencing offset: 0.27 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2M4F, Strand ID: A Detail

Release date

2013-05-12

Citation

Structural basis for complement evasion by Lyme disease pathogen Borrelia burgdorferi
Bhattacharjee, A., Oeemig, J.S., Kolodziejczyk, R., Meri, T., Kajander, T., Lehtinen, M.J., Iwai, H., Jokiranta, T., Goldman, A.
J. Biol. Chem. (2013), 288, 18685-18695, PubMed 23658013 , DOI 10.1074/jbc.M113.459040 , Abstract

Related entities 1. OspE, : 1 : 1 : 2 : 13 entities Detail

Experiments performed 19 experiments Detail

1 2D 1H-15N HSQC