BMRB: 19028

Flexible anchoring of archaeal MBF1 on ribosomes suggests role as recruitment factor

Authors

Launay, H., Blombarch, F., van des Oost, J., Christodoulou, J.

Assembly

MBF1-C

Entity

1. MBF1-C (polymer, Thiol state: not present), 106 monomers, 12165.65 Da Detail

ENAELEIVTD YYKIIKTARE QLGISQQQLA QKLKVSENIV KRFESGKLKP TISQARQLEK ILGIKLVTPL ENNEESEKEF DDTGLTLGDV VNIKEGKKLE HHHHHH

Formula weight

12165.65 Da

Source organism

Saccharolobus solfataricus

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 79.2 %, Completeness: 35.5 %, Completeness (bb): 64.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	35.5 % (457 of 1287)	19.0 % (129 of 678)	50.7 % (250 of 493)	67.2 % (78 of 116)
Backbone	64.7 % (409 of 632)	41.2 % (89 of 216)	77.6 % (242 of 312)	75.0 % (78 of 104)
Sidechain	16.7 % (126 of 755)	8.9 % (41 of 462)	30.2 % (85 of 281)	0.0 % (0 of 12)
Aromatic	0.0 % (0 of 60)	0.0 % (0 of 30)	0.0 % (0 of 30)
Methyl	14.8 % (19 of 128)	14.1 % (9 of 64)	15.6 % (10 of 64)

1. MBF1-C

ENAELEIVTD YYKIIKTARE QLGISQQQLA QKLKVSENIV KRFESGKLKP TISQARQLEK ILGIKLVTPL ENNEESEKEF DDTGLTLGDV VNIKEGKKLE HHHHHH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
1	MBF1-C	[U-100% 13C; U-100% 15N]		1 mM
2	sodium chloride	natural abundance		50 mM
3	D2O	natural abundance		10 %
4	H2O	natural abundance		90 %

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LACS Plot; CA

Referencing offset: -0.14 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.14 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.27 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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Protein Blocks Logo

Calculated from 16 models in PDB: 2MEZ, Strand ID: A Detail

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Release date

2014-06-04

Citation

Flexible anchoring of archeal MBF1 on ribosomes suggests role as recruitment factor
Launay, H., Blombarch, F., van des Oost, J., Christodoulou, J.
Nucleic Acids Res.

Related entities 1. MBF1-C, : 1 : 3 entities Detail

Experiments performed 5 experiments Detail