BMRB: 19035

G-rich VEGF aptamer with LNA modifications

Authors

Marusic, M., Veedu, R.N., Plavec, J.

Assembly

G-rich VEGF aptamer with LNA modifications

Entity

1. G-rich VEGF aptamer with LNA modifications (polymer, Thiol state: not present), 25 monomers, 8064.067 Da Detail

TGTGXGGGTG GACGGGCCGG XTAXA

Formula weight

8064.067 Da

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.0 %, Completeness: 76.4 %, Completeness (bb): 77.3 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H
All	76.4 % (159 of 208)	76.4 % (159 of 208)
Suger, PO4	77.3 % (119 of 154)	77.3 % (119 of 154)
Nucleobase	74.1 % (40 of 54)	74.1 % (40 of 54)
Aromatic	81.8 % (36 of 44)	81.8 % (36 of 44)
Methyl	100.0 % (4 of 4)	100.0 % (4 of 4)

1. DNA-RNA (25-MER)

TGTGXGGGTG GACGGGCCGG XTAXA

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	DNA/RNA (25-MER)	natural abundance		0.5 mM
2	potassium phosphate	natural abundance		10 mM
3	potassium chloride	natural abundance		90 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
6	DNA/RNA (25-MER)	natural abundance		0.5 mM
7	potassium phosphate	natural abundance		10 mM
8	potassium chloride	natural abundance		40 mM
9	H2O	natural abundance		90 %
10	D2O	natural abundance		10 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
11	DNA/RNA (25-MER)	natural abundance		0.5 mM
12	potassium phosphate	natural abundance		10 mM
13	potassium chloride	natural abundance		90 mM
14	H2O	natural abundance		90 %
15	D2O	natural abundance		10 %

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Release date

2013-08-13

Citation

G-rich VEGF aptamer with locked and unlocked nucleic acid modifications exhibits a unique G-quadruplex fold
Marusic, M., Veedu, R.N., Wengel, J., Plavec, J.
Nucleic Acids Res. (2013), 41, 9524-9536, PubMed 23935071 , DOI 10.1093/nar/gkt697 , Abstract

Experiments performed 6 experiments Detail