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BMRB: 19067

3ZPM

Solution structure of latherin

Authors

Vance, S.J., Macdonald, R.E., Cooper, A., Kennedy, M.W., Smith, B.O.

Assembly

Solution structure of latherin

Entity

1. Solution structure of latherin (polymer, Thiol state: all disulfide bound), 211 monomers, 22898.22 Da Detail

AMAQQIPPEV SSQITDALTQ GLLDGNFLSL LNAINLEGLL NTILDQVTGL LNILVGPLLG PSDAEIKLQD TRLLQLSLEF SPDSKGIDIW IPLELSVYLK LLILEPLTLY VRTDIRVQLR LESDEDGKYR LAFGHCSLLP RAIELQSGNP LSLPVNAVLG TIENALGNFI TEDLGAGLCP TLNSLVSNLD LQLVNNLINL ILDRANVDLS V

Formula weight

22898.22 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS136:SG	1:CYS179:SG

Source organism

Equus caballus

Exptl. method

solution NMR

Refine. method

AS PER PUBLICATION

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.6 %, Completeness: 95.9 %, Completeness (bb): 97.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	95.9 % (2336 of 2437)	95.4 % (1195 of 1253)	97.2 % (931 of 958)	92.9 % (210 of 226)
Backbone	97.3 % (1210 of 1244)	96.9 % (412 of 425)	97.7 % (605 of 619)	96.5 % (193 of 200)
Sidechain	95.1 % (1322 of 1390)	94.6 % (783 of 828)	97.4 % (522 of 536)	65.4 % (17 of 26)
Aromatic	92.5 % (74 of 80)	95.0 % (38 of 40)	89.7 % (35 of 39)	100.0 % (1 of 1)
Methyl	99.1 % (347 of 350)	98.9 % (173 of 175)	99.4 % (174 of 175)

1. LATHERIN

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 310.000 K, pH 7.500, Details Latherin 0.000500 M, NaCl 0.050000 M, NaPi 0.020000 M

#	Name	Isotope labeling	Concentration
1	LATHERIN	[U-13C; U-15N]	0.5 mM
2	sodium chloride	natural abundance	0.05 M
3	NaPi	natural abundance	0.02 M

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	internal	indirect	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	internal	indirect	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	internal	indirect	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	internal	indirect	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.24 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 3ZPM, Strand ID: A Detail

Release date

2013-06-23

Citation 1

The structure of latherin, a surfactant allergen protein from horse sweat and saliva
Vance, S.J., Macdonald, R.E., Cooper, A., Kennedy, M.W., Smith, B.O.
J. R. Soc. Interface. (2013), 10, 20130453-20130453, PubMed 23782536 , DOI 10.1098/rsif.2013.0453 , Abstract

Show more 2 citaions

Citation 2

Latherin: a surfactant protein of horse sweat and saliva
Mcdonald, R., Fleming, R., Beeley, J., Bovell, D., Lu, J., Zhao, X., Cooper, A., Kennedy, M.W.
PLoS One (2009), 4, e5726-e5726, PubMed 19478940 , DOI 10.1371/journal.pone.0005726 , Abstract

Citation 3

The Relationship between Structure and Function in Natural Surfactant Proteins
Vance, S.J.

Hide non-primary 2 citaions

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P82615 released on 2001-09-26
Title LATH_HORSE Entity Latherin

Related entities 1. Solution structure of latherin, : 1 : 1 : 15 entities Detail

Experiments performed 16 experiments Detail

1 15N-HSQC