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BMRB: 19068

2M5O

Solution NMR Structure CTD domain of NFU1 Iron-Sulfur Cluster Scaffold Homolog from Homo sapiens, Northeast Structural Genomics Consortium (NESG) Target HR2876C

Authors

Liu, G., Xiao, R., Janjua, H., Hamilton, K., Shastry, R., Kohan, E., Acton, T.B., Everett, J.K., Pederson, K., Huang, Y.J., Montelione, G.T.

Assembly

HR2876C

Entity

1. HR2876C (polymer, Thiol state: all free), 97 monomers, 10804.95 Da Detail

MGHHHHHHSH MGSEEDDGVV AMIKELLDTR IRPTVQEDGG DVIYKGFEDG IVQLKLQGSC TSCPSSIITL KNGIQNMLQF YIPEVEGVEQ VMDDESD

Formula weight

10804.95 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

distance geometry, simulated annealing, molecular dynamics, null

Data set

RDCs, assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 91.8 %, Completeness: 87.7 %, Completeness (bb): 87.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.7 % (953 of 1087)	87.6 % (493 of 563)	87.7 % (370 of 422)	88.2 % (90 of 102)
Backbone	87.3 % (503 of 576)	86.1 % (173 of 201)	88.3 % (248 of 281)	87.2 % (82 of 94)
Sidechain	87.6 % (524 of 598)	87.3 % (316 of 362)	87.7 % (200 of 228)	100.0 % (8 of 8)
Aromatic	59.4 % (38 of 64)	59.4 % (19 of 32)	59.4 % (19 of 32)
Methyl	99.0 % (97 of 98)	98.0 % (48 of 49)	100.0 % (49 of 49)

1. HR2876C

MGHHHHHHSH MGSEEDDGVV AMIKELLDTR IRPTVQEDGG DVIYKGFEDG IVQLKLQGSC TSCPSSIITL KNGIQNMLQF YIPEVEGVEQ VMDDESD

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR2876C.003, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	HR2876C.003	[U-100% 13C; U-100% 15N]	1.0 mM
2	DTT	natural abundance	5 mM
3	NaCl	natural abundance	100 mM
4	Tris-HCl pH 7.5	natural abundance	10 mM
5	NaN3	natural abundance	0.02 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR2876C.003, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
6	HR2876C.003	[U-100% 13C; U-100% 15N]	1.0 mM
7	DTT	natural abundance	5 mM
8	NaCl	natural abundance	100 mM
9	Tris-HCl pH 7.5	natural abundance	10 mM
10	NaN3	natural abundance	0.02 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR2876C.003, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
11	HR2876C.003	[U-100% 13C; U-100% 15N]	1.0 mM
12	DTT	natural abundance	5 mM
13	NaCl	natural abundance	100 mM
14	Tris-HCl pH 7.5	natural abundance	10 mM
15	NaN3	natural abundance	0.02 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.12 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2M5O, Strand ID: A Detail

RDC

140 RDC values in 2 lists
Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 298 K, pH 6.5 Detail

Release date

2013-04-28

Related entities 1. HR2876C, : 1 : 2 : 99 entities Detail

Interaction partners 1. HR2876C, : 15 interactors Detail

More details for 15 interactors identified by 8 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC