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BMRB: 19077

2MUU

1H, 13C and 15N backbone and side-chain resonance assignments of the N-terminal ubiquitin-binding domain of the human deubiquitinase USP28

Authors

Wen, Y., Cui, R., Zhang, H., Zhang, N.

Assembly

USP28 monomer

Entity

1. USP28 monomer (polymer, Thiol state: all free), 137 monomers, 14544.82 Da Detail

GSMTAELQQD DAAGAADGHG SSCQMLLNQL REITGIQDPS FLHEALKASN GDITQAVSLL TDERVKEPSQ DTVATEPSEV EGSAANKEVL AKVIDLTHDN KDDLQAAIAL SLLESPKIQA DGVDKLAAAL EHHHHHH

Formula weight

14544.82 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.8 %, Completeness: 83.9 %, Completeness (bb): 87.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	83.9 % (1233 of 1469)	83.1 % (626 of 753)	86.3 % (492 of 570)	78.8 % (115 of 146)
Backbone	87.2 % (710 of 814)	86.0 % (239 of 278)	88.6 % (357 of 403)	85.7 % (114 of 133)
Sidechain	80.2 % (629 of 784)	80.6 % (383 of 475)	82.8 % (245 of 296)	7.7 % (1 of 13)
Aromatic	0.0 % (0 of 46)	0.0 % (0 of 23)	0.0 % (0 of 23)
Methyl	89.9 % (151 of 168)	90.5 % (76 of 84)	89.3 % (75 of 84)

1. USP28

GSMTAELQQD DAAGAADGHG SSCQMLLNQL REITGIQDPS FLHEALKASN GDITQAVSLL TDERVKEPSQ DTVATEPSEV EGSAANKEVL AKVIDLTHDN KDDLQAAIAL SLLESPKIQA DGVDKLAAAL EHHHHHH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.2 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	D20	natural abundance	10 %
2	H20	natural abundance	90 %
3	USP-28	[U-15N]	0.8 ~ 1.0 mM
4	Na2HPO4/NaH2PO4	natural abundance	20 mM
5	NaCl	natural abundance	100 mM
6	DTT	natural abundance	2 mM
7	NaNH3	natural abundance	0.02 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.2 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	D20	natural abundance	10 %
9	H20	natural abundance	90 %
10	USP-28	[U-15N; U-13C]	0.8 ~ 1.0 mM
11	Na2HPO4/NaH2PO4	natural abundance	20 mM
12	NaCl	natural abundance	100 mM
13	DTT	natural abundance	2 mM
14	NaNH3	natural abundance	0.02 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.2 K, pH 6.5

#	Name	Isotope labeling	Concentration
15	D20	natural abundance	100 %
16	USP-28	[U-15N; U-13C]	0.8 ~ 1.0 mM
17	Na2HPO4/NaH2PO4	natural abundance	20 mM
18	NaCl	natural abundance	100 mM
19	DTT	natural abundance	2 mM
20	NaNH3	natural abundance	0.02 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	0.0 na	na	indirect	0.251
¹H	water	protons	4.77 na	internal	direct	1.0
¹⁵N	water	protons	0.0 na	na	indirect	0.101

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	0.0 na	na	indirect	0.251
¹H	water	protons	4.77 na	internal	direct	1.0
¹⁵N	water	protons	0.0 na	na	indirect	0.101

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	0.0 na	na	indirect	0.251
¹H	water	protons	4.77 na	internal	direct	1.0
¹⁵N	water	protons	0.0 na	na	indirect	0.101

Referencing offset: 0.03 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	0.0 na	na	indirect	0.251
¹H	water	protons	4.77 na	internal	direct	1.0
¹⁵N	water	protons	0.0 na	na	indirect	0.101

Referencing offset: 0.03 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 14 models in PDB: 2MUU, Strand ID: A Detail

Release date

2014-02-12

Citation

¹H, ¹³C and ¹⁵N backbone and side-chain resonance assignments of the N-terminal ubiquitin-binding domains of the human deubiquitinase Usp28
Wen, Y., Cui, R., Zhang, H., Zhang, N.
Biomol. NMR Assign. (2014), 8, 251-254, PubMed 23754699 , DOI 10.1007/s12104-013-9494-2 , Abstract

Related entities 1. USP28 monomer, : 2 : 5 entities Detail

Interaction partners 1. USP28 monomer, : 11 interactors Detail

More details for 11 interactors identified by 9 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC