BMRB: 19086

Solution structure of human ribosomal protein P1.P2 heterodimer

Authors

Lee, K., Yusa, K., Chu, L.O., Yu, C.W.H., Shaw, P.C., Oono, M., Miyoshi, T., Ito, K., Wong, K.B., Uchiumi, T.

Assembly

Solution structure of human ribosomal protein P1.P2 heterodimer

Entity

1. 60S ACIDIC RIBOSOMAL PROTEIN P1 (polymer, Thiol state: all free), 114 monomers, 11513.80 Da Detail

MASVSELACI YSALILHDDE VTVTEDKINA LIKAAGVNVE PFWPGLFAKA LANVNIGSLI CNVGAGGPAP AAGAAPAGGP APSTAAAPAE EKKVEAKKEE SEESDDDMGF GLFD

2. 60S ACIDIC RIBOSOMAL PROTEIN P2 (polymer, Thiol state: not present), 116 monomers, 11735.87 Da Detail

AMRYVASYLL AALGGNSSPS AKDIKKILDS VGIEADDDRL NKVISELNGK NIEDVIAQGI GKLASVPAGG AVAVSAAPGS AAPAAGSAPA AAEEKKDEKK EESEESDDDM GFGLFD

Total weight

23249.67 Da

Max. entity weight

11735.87 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

ARIA

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 50.0 %, Completeness: 34.5 %, Completeness (bb): 37.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C
All	34.5 % (753 of 2180)	42.1 % (519 of 1233)	24.7 % (234 of 947)
Backbone	37.4 % (425 of 1137)	45.5 % (214 of 470)	31.6 % (211 of 667)
Sidechain	33.4 % (418 of 1250)	40.0 % (305 of 763)	23.2 % (113 of 487)
Aromatic	16.2 % (16 of 99)	32.0 % (16 of 50)	0.0 % (0 of 49)
Methyl	34.3 % (92 of 268)	46.3 % (62 of 134)	22.4 % (30 of 134)

1. 60S ACIDIC RIBOSOMAL PROTEIN P1

MASVSELACI YSALILHDDE VTVTEDKINA LIKAAGVNVE PFWPGLFAKA LANVNIGSLI CNVGAGGPAP AAGAAPAGGP APSTAAAPAE EKKVEAKKEE SEESDDDMGF GLFD

2. 60S ACIDIC RIBOSOMAL PROTEIN P2

AMRYVASYLL AALGGNSSPS AKDIKKILDS VGIEADDDRL NKVISELNGK NIEDVIAQGI GKLASVPAGG AVAVSAAPGS AAPAAGSAPA AAEEKKDEKK EESEESDDDM GFGLFD

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Sample

Solvent system 10% water/90% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 1 mM

#	Name	Isotope labeling	Type	Concentration
1	60S ACIDIC RIBOSOMAL PROTEIN P1	[U-13C; U-15N]		1 mM
2	60S ACIDIC RIBOSOMAL PROTEIN P2	[U-13C; U-15N]		1 mM

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LACS Plot; CA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	indirect	1.0

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LACS Plot; CB

Referencing offset: -0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	indirect	1.0

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LACS Plot; HA

Referencing offset: 0.1 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	indirect	1.0

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Protein Blocks Logo

Calculated from 20 models in PDB: 4BEH, Strand ID: A, B Detail

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Release date

2013-03-11

Citation

Solution structure of human P1•P2 heterodimer provides insights into the role of eukaryotic stalk in recruiting the ribosome-inactivating protein trichosanthin to the ribosome
Lee, K.M., Yusa, K., Chu, L.O., Yu, C.W., Oono, M., Miyoshi, T., Ito, K., Shaw, P.C., Wong, K.B., Uchiumi, T.
Nucleic Acids Res. (2013), 41, 8776-8787, PubMed 23892290 , DOI 10.1093/nar/gkt636 , Abstract

Related entities 1. 60S ACIDIC RIBOSOMAL PROTEIN P1, : 1 : 4 : 1 : 61 entities Detail

Related entities 2. 60S ACIDIC RIBOSOMAL PROTEIN P2, : 1 : 3 : 83 entities Detail

Interaction partners 1. 60S ACIDIC RIBOSOMAL PROTEIN P1, : 62 : 1 interactors Detail

Interaction partners 2. 60S ACIDIC RIBOSOMAL PROTEIN P2, : 40 : 1 interactors Detail

Experiments performed 3 experiments Detail