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BMRB: 19098

2MZJ

Sidechain and backbone NMR resonance assignment for the RNA binding and C-terminal domain of the ribosome assembly factor Nop6 from S. cerevisiae

Authors

Wurm, J.P., Lioutikov, A., Koetter, P., Entian, K., Woehnert, J.

Assembly

Nop6

Entity

1. Nop6 (polymer, Thiol state: not present), 132 monomers, 14801.73 Da Detail

GNRFIVFVGS LPRDITAVEL QNHFKNSSPD QIRLRADKGI AFLEFDADKD RTGIQRRMDI ALLQHGTLLK EKKINVELTV GGGGNSQERL EKLKNKNIKL DEERKERLTK MINDGNQKKI AKTTATAAQT SG

Formula weight

14801.73 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.3 %, Completeness (bb): 99.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.3 % (1506 of 1564)	94.9 % (785 of 827)	98.6 % (583 of 591)	94.5 % (138 of 146)
Backbone	99.1 % (781 of 788)	98.2 % (268 of 273)	99.7 % (384 of 385)	99.2 % (129 of 130)
Sidechain	94.3 % (846 of 897)	93.3 % (517 of 554)	97.9 % (320 of 327)	56.3 % (9 of 16)
Aromatic	86.2 % (50 of 58)	86.2 % (25 of 29)	86.2 % (25 of 29)
Methyl	98.6 % (146 of 148)	97.3 % (72 of 74)	100.0 % (74 of 74)

1. Nop6

GNRFIVFVGS LPRDITAVEL QNHFKNSSPD QIRLRADKGI AFLEFDADKD RTGIQRRMDI ALLQHGTLLK EKKINVELTV GGGGNSQERL EKLKNKNIKL DEERKERLTK MINDGNQKKI AKTTATAAQT SG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	H2O	natural abundance	90 %
2	D2O	natural abundance	10 %
3	Nop6	U-15N	0.8 mM
4	KH2PO4	natural abundance	2 mM
5	Na2HPO4	natural abundance	8 mM
6	NaCl	natural abundance	100 mM
7	KCl	natural abundance	2.7 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Concentration
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %
10	Nop6	U-15N/U-13C	0.8 mM
11	KH2PO4	natural abundance	2 mM
12	Na2HPO4	natural abundance	8 mM
13	NaCl	natural abundance	100 mM
14	KCl	natural abundance	2.7 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.13 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MZJ, Strand ID: A Detail

Release date

2016-04-11

Citation

Backbone and sidechain NMR assignments for the ribosome assembly factor Nop6 from Saccharomyces cerevisiae
Wurm, J.P., Lioutikov, A., Koetter, P., Entian, K., Woehnert, J.
Biomol. NMR Assignments

Related entities 1. Nop6, : 2 : 8 entities Detail

Interaction partners 1. Nop6, : 30 interactors Detail

More details for 30 interactors identified by 12 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC