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BMRB: 19106

2M63

The protease-resistant N-terminal domain of TIR-domain containing adaptor molecule-1, TICAM-1

Authors

Kumeta, H., Enokizono, Y., Sakakibara, H., Ogura, K., Matsumoto, M., Seya, T., Inagaki, F.

Assembly

TICAM-1

Entity

1. TICAM-1 (polymer), 159 monomers, 17374.51 Da Detail

GPHMACTGPS LPSAFDILGA AGQDKLLYLK HKLKTPRPGC QGQDLLHAMV LLKLGQETEA RISLEALKAD AVARLVARQW AGVDSTEDPE EPPDVSWAVA RLYHLLAEEK LCPASLRDVA YQEAVRTLSS RDDHRLGELQ DEARNRCGWD IAGDPGSIR

Formula weight

17374.51 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

distance geometry, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 99.1 %, Completeness (bb): 99.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	99.1 % (1762 of 1778)	99.7 % (922 of 925)	98.1 % (680 of 693)	100.0 % (160 of 160)
Backbone	99.0 % (925 of 934)	99.4 % (318 of 320)	98.5 % (458 of 465)	100.0 % (149 of 149)
Sidechain	99.3 % (984 of 991)	99.8 % (604 of 605)	98.4 % (369 of 375)	100.0 % (11 of 11)
Aromatic	93.3 % (84 of 90)	100.0 % (45 of 45)	85.7 % (36 of 42)	100.0 % (3 of 3)
Methyl	100.0 % (186 of 186)	100.0 % (93 of 93)	100.0 % (93 of 93)

1. TICAM-1 NTD

GPHMACTGPS LPSAFDILGA AGQDKLLYLK HKLKTPRPGC QGQDLLHAMV LLKLGQETEA RISLEALKAD AVARLVARQW AGVDSTEDPE EPPDVSWAVA RLYHLLAEEK LCPASLRDVA YQEAVRTLSS RDDHRLGELQ DEARNRCGWD IAGDPGSIR

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	TICAM-1_NTD	[U-99% 13C; U-99% 15N]	0.75 mM
2	DTT	natural abundance	3 mM
3	sodium phosphate	natural abundance	50 mM
4	sodium azide	natural abundance	1 mM
5	DSS	natural abundance	0.02 mg/mL
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 5 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 5 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2M63, Strand ID: A Detail

Release date

2014-02-11

Citation

The N-terminal domain of TIR domain-containing adaptor molecule-1, TICAM-1
Kumeta, H., Sakakibara, H., Enokizono, Y., Ogura, K., Horiuchi, M., Matsumoto, M., Seya, T., Inagaki, F.
J. Biomol. NMR (2014), 58, 227-230, PubMed 24500696 , DOI 10.1007/s10858-014-9819-1 , Abstract

Related entities 1. TICAM-1, : 1 : 4 : 4 entities Detail

Interaction partners 1. TICAM-1, : 12 interactors Detail

More details for 12 interactors identified by 7 citations

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC