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BMRB: 19126

2M6B

Structure of full-length transmembrane domains of human glycine receptor 1 monomer subunit

Authors

Mowrey, D., Cui, T., Jia, Y., Ma, D., Makhov, A.M., Zhang, P., Tang, P., Xu, Y.

Assembly

GlyR

Entity

1. GlyR (polymer, Thiol state: all free), 150 monomers, 17299.14 Da Detail

MLERQLGYYL IQLYIPSLLI VILSWISFWI NLDAAPARVG LGITTVLTLT TQSSGSRASL PKVSYVKAID IWLAVCLLFV FSALLEYAAV NFVSRQREFG GGGFIQRAKK IDKISRIGFP LAFLIFNLFY WIIYKIVRRE DEFEHHHHHH

Formula weight

17299.14 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 54.8 %, Completeness (bb): 77.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	54.8 % (1019 of 1861)	59.0 % (563 of 954)	40.5 % (303 of 749)	96.8 % (153 of 158)
Backbone	77.1 % (688 of 892)	87.5 % (267 of 305)	62.4 % (275 of 441)	100.0 % (146 of 146)
Sidechain	40.0 % (444 of 1110)	45.6 % (296 of 649)	31.4 % (141 of 449)	58.3 % (7 of 12)
Aromatic	7.1 % (17 of 238)	14.3 % (17 of 119)	0.0 % (0 of 115)	0.0 % (0 of 4)
Methyl	44.1 % (97 of 220)	65.5 % (72 of 110)	22.7 % (25 of 110)

1. GlyR

MLERQLGYYL IQLYIPSLLI VILSWISFWI NLDAAPARVG LGITTVLTLT TQSSGSRASL PKVSYVKAID IWLAVCLLFV FSALLEYAAV NFVSRQREFG GGGFIQRAKK IDKISRIGFP LAFLIFNLFY WIIYKIVRRE DEFEHHHHHH

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.8

#	Name	Isotope labeling	Concentration
1	GlyR	natural abundance	480 uM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.8

#	Name	Isotope labeling	Concentration
4	GlyR	natural abundance	480 uM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.8

#	Name	Isotope labeling	Concentration
7	GlyR	natural abundance	480 uM
8	H2O	natural abundance	95 %
9	D2O	natural abundance	5 %

Sample #4

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.8

#	Name	Isotope labeling	Concentration
10	GlyR	natural abundance	480 uM
11	H2O	natural abundance	95 %
12	D2O	natural abundance	5 %

Sample #5

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.8

#	Name	Isotope labeling	Concentration
13	GlyR	natural abundance	480 uM
14	H2O	natural abundance	95 %
15	D2O	natural abundance	5 %

Sample #6

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.8

#	Name	Isotope labeling	Concentration
16	GlyR	natural abundance	480 uM
17	H2O	natural abundance	95 %
18	D2O	natural abundance	5 %

Sample #7

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.8

#	Name	Isotope labeling	Concentration
19	GlyR	natural abundance	480 uM
20	H2O	natural abundance	95 %
21	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.12 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 2M6B, Strand ID: A Detail

Release date

2013-09-02

Citation

Open-channel structures of the human glycine receptor α1 full-length transmembrane domain
Mowrey, D.D., Cui, T., Jia, Y., Ma, D., Makhov, A.M., Zhang, P., Tang, P., Xu, Y.
Structure (2013), 21, 1897-1904, PubMed 23994010 , DOI 10.1016/j.str.2013.07.014 , Abstract

Related entities 1. GlyR, : 1 : 1 : 123 entities Detail

Experiments performed 13 experiments Detail

1 3D 1H-15N NOESY