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BMRB: 19144

4BH9

A structural model of CAP mutant (T127L and S128I) in the apo state

Authors

Tzeng, S., Kalodimos, C.G.

Assembly

CAP mutant (T127L and S128I) in the apo state

Entity

1. CAP mutant (T127L and S128I) in the apo state (polymer, Thiol state: all free), 209 monomers, 23547.08 Da Detail

VLGKPQTDPT LEWFLSHCHI HKYPSKSTLI HQGEKAETLY YIVKGSVAVL IKDEEGKEMI LSYLNQGDFI GELGLFEEGQ ERSAWVRAKT ACEVAEISYK KFRQLIQVNP DILMRLSAQM ARRLQVLIEK VGNLAFLDVT GRIAQTLLNL AKQPDAMTHP DGMQIKITRQ EIGQIVGCSR ETVGRILKML EDQNLISAHG KTIVVYGTR

Formula weight

23547.08 Da

Source organism

Escherichia coli

Exptl. method

Refine. method

TORSION ANGLE DYNAMICS; SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 61.7 %, Completeness: 32.4 %, Completeness (bb): 45.5 % Detail

Polymer type: polypeptide(L)

	Total	¹³C	¹⁵N
All	32.4 % (386 of 1190)	26.6 % (257 of 966)	57.6 % (129 of 224)
Backbone	45.5 % (370 of 814)	39.4 % (241 of 611)	63.5 % (129 of 203)
Sidechain	4.7 % (27 of 569)	4.9 % (27 of 548)	0.0 % (0 of 21)
Aromatic	0.0 % (0 of 73)	0.0 % (0 of 71)	0.0 % (0 of 2)
Methyl	7.4 % (10 of 135)	7.4 % (10 of 135)

1. CAMP RECEPTOR PROTEIN

VLGKPQTDPT LEWFLSHCHI HKYPSKSTLI HQGEKAETLY YIVKGSVAVL IKDEEGKEMI LSYLNQGDFI GELGLFEEGQ ERSAWVRAKT ACEVAEISYK KFRQLIQVNP DILMRLSAQM ARRLQVLIEK VGNLAFLDVT GRIAQTLLNL AKQPDAMTHP DGMQIKITRQ EIGQIVGCSR ETVGRILKML EDQNLISAHG KTIVVYGTR

Show sample condition

Sample

Solvent system 10% water/90% D2O, Pressure 1.000 atm, Temperature 305.000 K, pH 6.000, Details 0.2mM

#	Name	Isotope labeling	Type	Concentration
1	CAMP RECEPTOR PROTEIN	[U-13C; U-15N]		0.2 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 4BH9, Strand ID: A Detail

Release date

2013-05-29

Citation

Allosteric inhibition through suppression of transient conformational states
Tzeng, S., Kalodimos, C.G.
Nat. Chem. Biol. (2013), 9, 462-465, PubMed 23644478 , DOI 10.1038/nchembio.1250 , Abstract

Related entities 1. CAP mutant (T127L and S128I) in the apo state, : 1 : 1 : 47 : 87 entities Detail

Experiments performed 12 experiments Detail

1 HNCA

Instrument

Bruker AvanceIII - 700 MHz

Sample

State isotropic, Solvent system 10% water/90% D2O, Pressure 1.000 atm, Temperature 305.000 K, pH 6.000, Details 0.2mM

#	Name	Isotope labeling	Type	Concentration
1	CAMP RECEPTOR PROTEIN	[U-13C; U-15N]		0.2 mM

2 HNCACB

Instrument

Bruker AvanceIII - 700 MHz

Sample

State isotropic, Solvent system 10% water/90% D2O, Pressure 1.000 atm, Temperature 305.000 K, pH 6.000, Details 0.2mM

#	Name	Isotope labeling	Type	Concentration
1	CAMP RECEPTOR PROTEIN	[U-13C; U-15N]		0.2 mM

3 HNCO

Instrument

Bruker AvanceIII - 700 MHz

Sample

State isotropic, Solvent system 10% water/90% D2O, Pressure 1.000 atm, Temperature 305.000 K, pH 6.000, Details 0.2mM

#	Name	Isotope labeling	Type	Concentration
1	CAMP RECEPTOR PROTEIN	[U-13C; U-15N]		0.2 mM

4 HNCOCA

Instrument

Bruker AvanceIII - 700 MHz

Sample

State isotropic, Solvent system 10% water/90% D2O, Pressure 1.000 atm, Temperature 305.000 K, pH 6.000, Details 0.2mM

#	Name	Isotope labeling	Type	Concentration
1	CAMP RECEPTOR PROTEIN	[U-13C; U-15N]		0.2 mM

5 3D-15N-NOESY

Instrument

Bruker AvanceIII - 700 MHz

Sample

State isotropic, Solvent system 10% water/90% D2O, Pressure 1.000 atm, Temperature 305.000 K, pH 6.000, Details 0.2mM

#	Name	Isotope labeling	Type	Concentration
1	CAMP RECEPTOR PROTEIN	[U-13C; U-15N]		0.2 mM

6 3D-13C-NOESY

Instrument

Bruker AvanceIII - 700 MHz

Sample

State isotropic, Solvent system 10% water/90% D2O, Pressure 1.000 atm, Temperature 305.000 K, pH 6.000, Details 0.2mM

#	Name	Isotope labeling	Type	Concentration
1	CAMP RECEPTOR PROTEIN	[U-13C; U-15N]		0.2 mM

7 HNCA

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 10% water/90% D2O, Pressure 1.000 atm, Temperature 305.000 K, pH 6.000, Details 0.2mM

#	Name	Isotope labeling	Type	Concentration
1	CAMP RECEPTOR PROTEIN	[U-13C; U-15N]		0.2 mM

8 HNCACB

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 10% water/90% D2O, Pressure 1.000 atm, Temperature 305.000 K, pH 6.000, Details 0.2mM

#	Name	Isotope labeling	Type	Concentration
1	CAMP RECEPTOR PROTEIN	[U-13C; U-15N]		0.2 mM

9 HNCO

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 10% water/90% D2O, Pressure 1.000 atm, Temperature 305.000 K, pH 6.000, Details 0.2mM

#	Name	Isotope labeling	Type	Concentration
1	CAMP RECEPTOR PROTEIN	[U-13C; U-15N]		0.2 mM

10 HNCOCA

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 10% water/90% D2O, Pressure 1.000 atm, Temperature 305.000 K, pH 6.000, Details 0.2mM

#	Name	Isotope labeling	Type	Concentration
1	CAMP RECEPTOR PROTEIN	[U-13C; U-15N]		0.2 mM

11 3D-15N-NOESY

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 10% water/90% D2O, Pressure 1.000 atm, Temperature 305.000 K, pH 6.000, Details 0.2mM

#	Name	Isotope labeling	Type	Concentration
1	CAMP RECEPTOR PROTEIN	[U-13C; U-15N]		0.2 mM

12 3D-13C-NOESY

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 10% water/90% D2O, Pressure 1.000 atm, Temperature 305.000 K, pH 6.000, Details 0.2mM

#	Name	Isotope labeling	Type	Concentration
1	CAMP RECEPTOR PROTEIN	[U-13C; U-15N]		0.2 mM

nullKeywords cAMP receptor protein, Catabolite Activator Protein, TRANSCRIPTION