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BMRB: 19148

2M6O

The actinobacterial transcription factor RbpA binds to the principal sigma subunit of RNA polymerase

Authors

Liu, B., Tabib-Salazar1, A., Doughty, P., Lewis, R., Ghosh, S., Parsy, M., Simpson, P., O'Dwyer, K., Matthews, S., Paget, M.

Assembly

actinobacterial transcription factor RbpA

Entity

1. actinobacterial transcription factor RbpA (polymer), 48 monomers, 5351.966 Da Detail

QAVEYACEKG HRFEMPFSVE AEIPPEWECK VCGAQALLVD GDGPEEKK

Formula weight

5351.966 Da

Source organism

Escherichia coli

Exptl. method

Refine. method

simulated annealing, distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.9 %, Completeness: 86.1 %, Completeness (bb): 97.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.1 % (472 of 548)	83.0 % (239 of 288)	88.7 % (189 of 213)	93.6 % (44 of 47)
Backbone	97.9 % (274 of 280)	96.9 % (93 of 96)	97.9 % (137 of 140)	100.0 % (44 of 44)
Sidechain	77.2 % (241 of 312)	76.0 % (146 of 192)	81.2 % (95 of 117)	0.0 % (0 of 3)
Aromatic	59.1 % (26 of 44)	59.1 % (13 of 22)	61.9 % (13 of 21)	0.0 % (0 of 1)
Methyl	100.0 % (38 of 38)	100.0 % (19 of 19)	100.0 % (19 of 19)

1. entity

QAVEYACEKG HRFEMPFSVE AEIPPEWECK VCGAQALLVD GDGPEEKK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	beta-mercaptoethanol	natural abundance		3 mM
2	sodium chloride	natural abundance		500 mM
3	TRIS	natural abundance		20 mM
4	RbpA	natural abundance		0.0 ~ 0.0 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

LACS Plot; CA

Referencing offset: -0.27 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: -0.27 ppm, Outliers: 1 Detail

LACS Plot; HA

Referencing offset: 0.06 ppm, Outliers: 1 Detail

LACS Plot; CO

Referencing offset: 0.48 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2M6O, Strand ID: A Detail

Release date

2013-05-05

Citation

The actinobacterial transcription factor RbpA binds to the principal sigma subunit of RNA polymerase
Tabib-Salazar1, A., Liu, B., Doughty, P., Lewis, R., Ghosh, S., Parsy, M., Simpson, P., O'Dwyer, K., Matthews, S., Paget, M.
Nucleic Acids Res. (2013), 41, 5679-5691, PubMed 23605043 , DOI 10.1093/nar/gkt277 , Abstract

Related entities 1. actinobacterial transcription factor RbpA, : 1 : 1 : 34 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	beta-mercaptoethanol	natural abundance		3 mM
2	sodium chloride	natural abundance		500 mM
3	TRIS	natural abundance		20 mM
4	RbpA	natural abundance		0.0 ~ 0.0 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

2 3D HNCO

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	beta-mercaptoethanol	natural abundance		3 mM
2	sodium chloride	natural abundance		500 mM
3	TRIS	natural abundance		20 mM
4	RbpA	natural abundance		0.0 ~ 0.0 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

3 3D HNCA

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	beta-mercaptoethanol	natural abundance		3 mM
2	sodium chloride	natural abundance		500 mM
3	TRIS	natural abundance		20 mM
4	RbpA	natural abundance		0.0 ~ 0.0 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

4 3D HNCACB

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	beta-mercaptoethanol	natural abundance		3 mM
2	sodium chloride	natural abundance		500 mM
3	TRIS	natural abundance		20 mM
4	RbpA	natural abundance		0.0 ~ 0.0 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

5 3D CBCA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	beta-mercaptoethanol	natural abundance		3 mM
2	sodium chloride	natural abundance		500 mM
3	TRIS	natural abundance		20 mM
4	RbpA	natural abundance		0.0 ~ 0.0 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

6 3D HBHA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	beta-mercaptoethanol	natural abundance		3 mM
2	sodium chloride	natural abundance		500 mM
3	TRIS	natural abundance		20 mM
4	RbpA	natural abundance		0.0 ~ 0.0 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

7 3D 1H-13C NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	beta-mercaptoethanol	natural abundance		3 mM
2	sodium chloride	natural abundance		500 mM
3	TRIS	natural abundance		20 mM
4	RbpA	natural abundance		0.0 ~ 0.0 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

8 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	beta-mercaptoethanol	natural abundance		3 mM
2	sodium chloride	natural abundance		500 mM
3	TRIS	natural abundance		20 mM
4	RbpA	natural abundance		0.0 ~ 0.0 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

9 3D HCCH-TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	beta-mercaptoethanol	natural abundance		3 mM
2	sodium chloride	natural abundance		500 mM
3	TRIS	natural abundance		20 mM
4	RbpA	natural abundance		0.0 ~ 0.0 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

nullKeywords RbpA, RNAP, Sigma factor