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BMRB: 19149

2M6P

The actinobacterial transcription factor RbpA binds to the principal sigma subunit of RNA polymerase

Authors

Liu, B., Parsy, M., O'Dwyer, K., Paget, M., Matthews, S.

Assembly

actinobacterial transcription factor RbpA

Entity

1. actinobacterial transcription factor RbpA (polymer), 46 monomers, 5209.648 Da Detail

PRQIARYRTD NGEEFEVPFA DDAEIPGTWL CRNGMEGTLI EGDLPE

Formula weight

5209.648 Da

Source organism

Escherichia coli

Exptl. method

Refine. method

distance geometry, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.8 %, Completeness: 92.3 %, Completeness (bb): 93.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.3 % (479 of 519)	91.9 % (250 of 272)	92.0 % (185 of 201)	95.7 % (44 of 46)
Backbone	93.7 % (251 of 268)	94.6 % (88 of 93)	92.5 % (123 of 133)	95.2 % (40 of 42)
Sidechain	89.0 % (260 of 292)	87.2 % (156 of 179)	91.7 % (100 of 109)	100.0 % (4 of 4)
Aromatic	97.5 % (39 of 40)	95.0 % (19 of 20)	100.0 % (19 of 19)	100.0 % (1 of 1)
Methyl	100.0 % (40 of 40)	100.0 % (20 of 20)	100.0 % (20 of 20)

1. RbpA

PRQIARYRTD NGEEFEVPFA DDAEIPGTWL CRNGMEGTLI EGDLPE

Show sample condition

Sample

Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	beta-mercaptoethanol	natural abundance		3 mM
2	sodium chloride	natural abundance		500 mM
3	TRIS	natural abundance		20 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %
6	RbpA	natural abundance		0.0 ~ 0.0 mM

LACS Plot; CA

Referencing offset: 0.03 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: 0.03 ppm, Outliers: 1 Detail

LACS Plot; HA

Referencing offset: 0.09 ppm, Outliers: 2 Detail

LACS Plot; CO

Referencing offset: 0.55 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2M6P, Strand ID: A Detail

Release date

2013-05-05

Citation

The actinobacterial transcription factor RbpA binds to the principal sigma subunit of RNA polymerase
Tabib-Salazar, A., Liu, B., Doughty, P., Lewis, R., Ghosh, S., Parsy, M., O'Dwyer, K., Simpson, P., Matthews, S., Paget, M.
Nucleic Acids Res. (2013), 41, 5679-5691, PubMed 23605043 , DOI 10.1093/nar/gkt277 , Abstract

Related entities 1. actinobacterial transcription factor RbpA, : 1 : 19 : 11 : 6 entities Detail

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	beta-mercaptoethanol	natural abundance		3 mM
2	sodium chloride	natural abundance		500 mM
3	TRIS	natural abundance		20 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %
6	RbpA	natural abundance		0.0 ~ 0.0 mM

2 3D C(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	beta-mercaptoethanol	natural abundance		3 mM
2	sodium chloride	natural abundance		500 mM
3	TRIS	natural abundance		20 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %
6	RbpA	natural abundance		0.0 ~ 0.0 mM

3 3D CBCA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	beta-mercaptoethanol	natural abundance		3 mM
2	sodium chloride	natural abundance		500 mM
3	TRIS	natural abundance		20 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %
6	RbpA	natural abundance		0.0 ~ 0.0 mM

4 3D C(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	beta-mercaptoethanol	natural abundance		3 mM
2	sodium chloride	natural abundance		500 mM
3	TRIS	natural abundance		20 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %
6	RbpA	natural abundance		0.0 ~ 0.0 mM

5 3D HNCO

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	beta-mercaptoethanol	natural abundance		3 mM
2	sodium chloride	natural abundance		500 mM
3	TRIS	natural abundance		20 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %
6	RbpA	natural abundance		0.0 ~ 0.0 mM

6 3D HNCA

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	beta-mercaptoethanol	natural abundance		3 mM
2	sodium chloride	natural abundance		500 mM
3	TRIS	natural abundance		20 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %
6	RbpA	natural abundance		0.0 ~ 0.0 mM

7 3D HNCACB

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	beta-mercaptoethanol	natural abundance		3 mM
2	sodium chloride	natural abundance		500 mM
3	TRIS	natural abundance		20 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %
6	RbpA	natural abundance		0.0 ~ 0.0 mM

8 3D HCCH-TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	beta-mercaptoethanol	natural abundance		3 mM
2	sodium chloride	natural abundance		500 mM
3	TRIS	natural abundance		20 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %
6	RbpA	natural abundance		0.0 ~ 0.0 mM

9 3D HBHA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	beta-mercaptoethanol	natural abundance		3 mM
2	sodium chloride	natural abundance		500 mM
3	TRIS	natural abundance		20 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %
6	RbpA	natural abundance		0.0 ~ 0.0 mM

10 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	beta-mercaptoethanol	natural abundance		3 mM
2	sodium chloride	natural abundance		500 mM
3	TRIS	natural abundance		20 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %
6	RbpA	natural abundance		0.0 ~ 0.0 mM

11 3D 1H-13C NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	beta-mercaptoethanol	natural abundance		3 mM
2	sodium chloride	natural abundance		500 mM
3	TRIS	natural abundance		20 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %
6	RbpA	natural abundance		0.0 ~ 0.0 mM

nullKeywords MTB, mv2050, RNAP, Sigma factor