holo_YqcA
MAEIGIFVGT MYGNSLLVAE EAEAILTAQG HKATVFEDPE LSDWLPYQDK YVLVVTSTTG QGDLPDSIVP LFQGIKDSLG FQPNLRYGVI ALGDSSYVNF CNGGKQFDAL LQEQSAQRVG EMLLIDASEN PEPETESNPW VEQWGTLLS
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 92.3 % (1563 of 1693) | 90.8 % (790 of 870) | 93.2 % (618 of 663) | 96.9 % (155 of 160) |
Backbone | 97.6 % (857 of 878) | 97.0 % (295 of 304) | 97.9 % (424 of 433) | 97.9 % (138 of 141) |
Sidechain | 88.2 % (838 of 950) | 87.5 % (495 of 566) | 89.3 % (326 of 365) | 89.5 % (17 of 19) |
Aromatic | 58.6 % (82 of 140) | 57.1 % (40 of 70) | 58.2 % (39 of 67) | 100.0 % (3 of 3) |
Methyl | 94.9 % (167 of 176) | 94.3 % (83 of 88) | 95.5 % (84 of 88) |
1. holo YqcA
MAEIGIFVGT MYGNSLLVAE EAEAILTAQG HKATVFEDPE LSDWLPYQDK YVLVVTSTTG QGDLPDSIVP LFQGIKDSLG FQPNLRYGVI ALGDSSYVNF CNGGKQFDAL LQEQSAQRVG EMLLIDASEN PEPETESNPW VEQWGTLLSSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | holo_YqcA | [U-15N] | 1 mM | |
2 | sodium phosphate | natural abundance | 30 mM | |
3 | FLAVIN MONONUCLEOTIDE | natural abundance | 50 mM | |
4 | sodium chloride | natural abundance | 30 mM | |
5 | H2O | natural abundance | 90 % | |
6 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | holo_YqcA | [U-13C; U-15N] | 1 mM | |
8 | sodium phosphate | natural abundance | 30 mM | |
9 | FLAVIN MONONUCLEOTIDE | natural abundance | 50 mM | |
10 | sodium chloride | natural abundance | 30 mM | |
11 | H2O | natural abundance | 90 % | |
12 | D2O | natural abundance | 10 % |
Bruker Avance - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | holo_YqcA | [U-15N] | 1 mM | |
2 | sodium phosphate | natural abundance | 30 mM | |
3 | FLAVIN MONONUCLEOTIDE | natural abundance | 50 mM | |
4 | sodium chloride | natural abundance | 30 mM | |
5 | H2O | natural abundance | 90 % | |
6 | D2O | natural abundance | 10 % |
Bruker Avance - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | holo_YqcA | [U-13C; U-15N] | 1 mM | |
8 | sodium phosphate | natural abundance | 30 mM | |
9 | FLAVIN MONONUCLEOTIDE | natural abundance | 50 mM | |
10 | sodium chloride | natural abundance | 30 mM | |
11 | H2O | natural abundance | 90 % | |
12 | D2O | natural abundance | 10 % |
Bruker Avance - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | holo_YqcA | [U-13C; U-15N] | 1 mM | |
8 | sodium phosphate | natural abundance | 30 mM | |
9 | FLAVIN MONONUCLEOTIDE | natural abundance | 50 mM | |
10 | sodium chloride | natural abundance | 30 mM | |
11 | H2O | natural abundance | 90 % | |
12 | D2O | natural abundance | 10 % |
Bruker Avance - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | holo_YqcA | [U-13C; U-15N] | 1 mM | |
8 | sodium phosphate | natural abundance | 30 mM | |
9 | FLAVIN MONONUCLEOTIDE | natural abundance | 50 mM | |
10 | sodium chloride | natural abundance | 30 mM | |
11 | H2O | natural abundance | 90 % | |
12 | D2O | natural abundance | 10 % |
Bruker Avance - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | holo_YqcA | [U-13C; U-15N] | 1 mM | |
8 | sodium phosphate | natural abundance | 30 mM | |
9 | FLAVIN MONONUCLEOTIDE | natural abundance | 50 mM | |
10 | sodium chloride | natural abundance | 30 mM | |
11 | H2O | natural abundance | 90 % | |
12 | D2O | natural abundance | 10 % |
Bruker Avance - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | holo_YqcA | [U-13C; U-15N] | 1 mM | |
8 | sodium phosphate | natural abundance | 30 mM | |
9 | FLAVIN MONONUCLEOTIDE | natural abundance | 50 mM | |
10 | sodium chloride | natural abundance | 30 mM | |
11 | H2O | natural abundance | 90 % | |
12 | D2O | natural abundance | 10 % |
Bruker Avance - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | holo_YqcA | [U-13C; U-15N] | 1 mM | |
8 | sodium phosphate | natural abundance | 30 mM | |
9 | FLAVIN MONONUCLEOTIDE | natural abundance | 50 mM | |
10 | sodium chloride | natural abundance | 30 mM | |
11 | H2O | natural abundance | 90 % | |
12 | D2O | natural abundance | 10 % |
Bruker Avance - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | holo_YqcA | [U-15N] | 1 mM | |
2 | sodium phosphate | natural abundance | 30 mM | |
3 | FLAVIN MONONUCLEOTIDE | natural abundance | 50 mM | |
4 | sodium chloride | natural abundance | 30 mM | |
5 | H2O | natural abundance | 90 % | |
6 | D2O | natural abundance | 10 % |
Bruker Avance - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | holo_YqcA | [U-13C; U-15N] | 1 mM | |
8 | sodium phosphate | natural abundance | 30 mM | |
9 | FLAVIN MONONUCLEOTIDE | natural abundance | 50 mM | |
10 | sodium chloride | natural abundance | 30 mM | |
11 | H2O | natural abundance | 90 % | |
12 | D2O | natural abundance | 10 % |
Properties
Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints | combined_19152_2m6s.nef |
Input source #2: Coordindates | 2m6s.cif |
Diamagnetism of the molecular assembly | True (excluding Oxygen atoms) |
Whether the assembly has a disulfide bond | None |
Whether the assembly has a other bond | None |
Whether the assembly contains a cyclic polymer | None |
Overall data processing status | Error |
Disulfide bonds
NoneOther bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)
NoneNon-standard residues
Chain_ID | Seq_ID | Comp_ID | Chem_comp_name | Experimental evidences |
---|---|---|---|---|
B | 1 | FMN | FLAVIN MONONUCLEOTIDE | None |
Sequence alignments
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDWLPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNF |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDWLPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNF -------110-------120-------130-------140--------- CNGGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTLLS ||||||||||||||||||||||||||||||||||||||||||||||||| CNGGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTLLS
Chain assignments
Entity_assembly_ID (NMR) | Auth_asym_ID (model) | Length | Unmapped | Conflict | Sequence coverage (%) |
---|---|---|---|---|---|
A | A | 149 | 0 | 0 | 100.0 |
Content subtype: combined_19152_2m6s.nef
Assigned chemical shifts
Distance restraints
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDWLPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNF ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| .AEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDWLPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNF -------110-------120-------130-------140--------- CNGGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTLLS ||||||||||||||||||||||||||||||||||||||||||||||||| CNGGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTLLS