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Structure of the p7 channel of Hepatitis C virus, genotype 5a
Authors
OuYang, B., Chou, J.J.
Assembly
p7 channel of Hepatitis C virus, genotype 5a
Entity
1. p7 channel of Hepatitis C virus, genotype 5a (polymer, Thiol state: not present), 63 monomers, 6752.999 × 6 Da Detail

GAKNVIVLNA ASAAGNHGFF WGLLVVTLAW HVKGRLVPGA TYLSLGVWPL LLVRLLRPHR ALA


Total weight
40517.992 Da
Max. entity weight
6752.999 Da
Source organism
Hepacivirus C
Exptl. method
solution NMR
Refine. method
simulated annealing, molecular dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 98.4 %, Completeness: 56.9 %, Completeness (bb): 81.2 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All56.9 % (418 of 734)36.1 % (134 of 371)76.1 % (226 of 297)87.9 % (58 of 66)
Backbone81.2 % (302 of 372)50.0 % (65 of 130)98.4 % (179 of 182)96.7 % (58 of 60)
Sidechain40.9 % (171 of 418)28.6 % (69 of 241)59.6 % (102 of 171) 0.0 % (0 of 6)
Aromatic 0.0 % (0 of 76) 0.0 % (0 of 38) 0.0 % (0 of 35) 0.0 % (0 of 3)
Methyl97.3 % (107 of 110)96.4 % (53 of 55)98.2 % (54 of 55)

1. HCV p7 hexamer

GAKNVIVLNA ASAAGNHGFF WGLLVVTLAW HVKGRLVPGA TYLSLGVWPL LLVRLLRPHR ALA

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5


#NameIsotope labelingTypeConcentration
1HCV p7 (monomer)[U-100% 13C; U-100% 15N; U-80% 2H]0.8 mM
2DPCnatural abundance200 mM
3MESnatural abundance25 mM
4H2Onatural abundance95 %
5D2Onatural abundance5 %
Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5


#NameIsotope labelingTypeConcentration
6HCV p7 (monomer)[U-100% 13C; U-100% 15N]0.8 mM
7DPC[U-2H]200 mM
8MESnatural abundance25 mM
9H2Onatural abundance95 %
10D2Onatural abundance5 %
Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5


#NameIsotope labelingTypeConcentration
11HCV p7 (monomer)[U-15% 13C]0.8 mM
12DPC[U-2H]200 mM
13MESnatural abundance25 mM
14H2Onatural abundance95 %
15D2Onatural abundance5 %
Sample #4

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5


#NameIsotope labelingTypeConcentration
16HCV p7 (monomer)[U-15N; U-2H]0.4 mM
17HCV p7 (monomer)[U-13C]0.4 mM
18DPC[U-2H]200 mM
19MESnatural abundance25 mM
20H2Onatural abundance95 %
21D2Onatural abundance5 %
Sample #5

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5


#NameIsotope labelingTypeConcentration
22HCV p7 (monomer)[U-100% 13C; U-100% 15N; U-80% 2H]0.2 mM
23DPCnatural abundance100 mM
24MESnatural abundance25 mM
25polyacrylamide gelnatural abundance4.5 %
26H2Onatural abundance95 %
27D2Onatural abundance5 %

LACS Plot; CA
Referencing offset: -0.04 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.04 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.11 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 15 models in PDB: 2M6X, Strand ID: A, B, C, D, E, F Detail


Release date
2013-04-28
Citation
Unusual architecture of the p7 channel from hepatitis C virus
OuYang, B., Xie, S., Berardi, M.J., Zhao, X., Dev, J., Yu, W., Sun, B., Chou, J.J.
Nature (2013), 498, 521-525, PubMed 23739335 , DOI 10.1038/nature12283 ,
Related entities 1. p7 channel of Hepatitis C virus, genotype 5a, : 1 : 30 entities Detail
Experiments performed 12 experiments Detail
NMR combined restraints 6 contents Detail
Keywords HCV, cation channel, hexamer, membrane protein, p7