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BMRB: 19178

2M7A

Enteropathogenic Escherichia coli 0111:H- str. 11128 ORF EC0111_1119 similar to bacteriophage lambda ea8.5

Authors

Donaldson, L.W.

Assembly

MS1

Entity

1. MS1 (polymer, Thiol state: all free), 99 monomers, 11171.34 Da Detail

MKRGVEMSIH DLCEDQEQWA MQTLMGSGVL ARCRIHNDVI LDSGNDASSA YKLGTYLYQK DNSCNLFNTL TEARDAIKDA YESYCGIDDC PQCSKYIDD

2. ZINC ION (non-polymer, Thiol state: not present), 65.409 Da

Total weight

11236.749 Da

Max. entity weight

11171.34 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 88.9 %, Completeness: 72.6 %, Completeness (bb): 78.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	72.6 % (802 of 1105)	70.3 % (402 of 572)	73.3 % (311 of 424)	81.7 % (89 of 109)
Backbone	78.7 % (466 of 592)	81.3 % (165 of 203)	74.9 % (218 of 291)	84.7 % (83 of 98)
Sidechain	66.3 % (402 of 606)	64.2 % (237 of 369)	70.4 % (159 of 226)	54.5 % (6 of 11)
Aromatic	89.7 % (70 of 78)	97.4 % (38 of 39)	81.6 % (31 of 38)	100.0 % (1 of 1)
Methyl	95.6 % (86 of 90)	95.6 % (43 of 45)	95.6 % (43 of 45)

1. MS1

MKRGVEMSIH DLCEDQEQWA MQTLMGSGVL ARCRIHNDVI LDSGNDASSA YKLGTYLYQK DNSCNLFNTL TEARDAIKDA YESYCGIDDC PQCSKYIDD

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 289.000 K, pH 7.800

#	Name	Isotope labeling	Concentration
1	MS1	[U-99% 13C; U-99% 15N]	0.6 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.42 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.42 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2M7A, Strand ID: A Detail

Release date

2013-06-09

Citation

The solution structures of two prophage homologues of the bacteriophage λ Ea8.5 protein reveal a newly discovered hybrid homeodomain/zinc-finger fold
Kwan, J.J., Smirnova, E., Khazai, S., Evanics, F., Maxwell, K.L., Donaldson, L.W.
Biochemistry (2013), 52, 3612-3614, PubMed 23672713 , DOI 10.1021/bi400543w , Abstract

Related entities 1. MS1, : 1 : 6 entities Detail

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC/HMQC