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BMRB: 19179

2M7B

ORF PP_3909 from Pseudomonas putida KT2440 encoding a protein similar to bacteriophage lambda ea8.5

Authors

Donaldson, L.W.

Assembly

mol1

Entity

1. mol1 (polymer, Thiol state: all free), 92 monomers, 10214.10 Da Detail

MGGIKRLMEE EDAKYSEAVY IAIEAGTLAE CEVHEGTYFS DSGDISEAEE LAREKFEKGE VSNFDDVEEL VKKVVAVCEE LGAEECFSCD FD

2. ZINC ION (non-polymer, Thiol state: not present), 65.409 Da

Total weight

10279.509 Da

Max. entity weight

10214.1 Da

Source organism

Pseudomonas putida

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 87.0 %, Completeness: 77.3 %, Completeness (bb): 81.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	77.3 % (782 of 1012)	76.2 % (396 of 520)	77.9 % (311 of 399)	80.6 % (75 of 93)
Backbone	81.0 % (447 of 552)	79.1 % (151 of 191)	82.5 % (222 of 269)	80.4 % (74 of 92)
Sidechain	74.5 % (406 of 545)	74.5 % (245 of 329)	74.4 % (160 of 215)	100.0 % (1 of 1)
Aromatic	76.9 % (60 of 78)	87.2 % (34 of 39)	66.7 % (26 of 39)
Methyl	92.2 % (83 of 90)	93.3 % (42 of 45)	91.1 % (41 of 45)

1. mol1

MGGIKRLMEE EDAKYSEAVY IAIEAGTLAE CEVHEGTYFS DSGDISEAEE LAREKFEKGE VSNFDDVEEL VKKVVAVCEE LGAEECFSCD FD

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 7.800

#	Name	Isotope labeling	Concentration
1	mol1	[U-99% 13C; U-99% 15N]	0.6 mM
2	H20	natural abundance	90 %
3	D20	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 6 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2M7B, Strand ID: A Detail

Release date

2013-06-09

Citation

The solution structures of two prophage homologues of the bacteriophage λ Ea8.5 protein reveal a newly discovered hybrid homeodomain/zinc-finger fold
Kwan, J.J., Smirnova, E., Khazai, S., Evanics, F., Maxwell, K.L., Donaldson, L.W.
Biochemistry (2013), 52, 3612-3614, PubMed 23672713 , DOI 10.1021/bi400543w , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 19178 released on 2013-06-09
Title Enteropathogenic Escherichia coli 0111:H- str. 11128 ORF EC0111_1119 similar to bacteriophage lambda ea8.5

Related entities 1. mol1, : 1 : 1 entities Detail

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC/HMQC