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BMRB: 19195

2M7L

alfa-actinin from parasite Entamoeba histolytica

Authors

Persson, C.K., Mayzel, M.L., Karlsson, G.B., Backman, L.

Assembly

alfa-actinin from parasite Entamoeba histolytica

Entity

1. alfa-actinin from parasite Entamoeba histolytica (polymer, Thiol state: all free), 144 monomers, 15912.12 Da Detail

GSSSGVTAEQ MQEFKQSFDA FDGNHDGILD KLEFRSCLSS MGLIDIDFTG GEDAQYDAIY NNVTKGENGV SFDNYVQYMK EKNDENPSPE QLNEIFSTIA AGKDSITETD MQKAGMSAEQ IEYVKANLPQ KGDGYDYAAW VKTN

Formula weight

15912.12 Da

Source organism

Entamoeba histolytica

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 89.8 %, Completeness (bb): 94.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.8 % (1454 of 1620)	90.5 % (760 of 840)	89.2 % (552 of 619)	88.2 % (142 of 161)
Backbone	94.1 % (807 of 858)	95.6 % (285 of 298)	92.1 % (386 of 419)	96.5 % (136 of 141)
Sidechain	86.8 % (775 of 893)	87.5 % (474 of 542)	89.4 % (296 of 331)	25.0 % (5 of 20)
Aromatic	77.5 % (110 of 142)	93.0 % (66 of 71)	61.4 % (43 of 70)	100.0 % (1 of 1)
Methyl	100.0 % (116 of 116)	100.0 % (58 of 58)	100.0 % (58 of 58)

1. entity

GSSSGVTAEQ MQEFKQSFDA FDGNHDGILD KLEFRSCLSS MGLIDIDFTG GEDAQYDAIY NNVTKGENGV SFDNYVQYMK EKNDENPSPE QLNEIFSTIA AGKDSITETD MQKAGMSAEQ IEYVKANLPQ KGDGYDYAAW VKTN

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 25mM NaPi, 150 mM NaCl, pH 6.0

#	Name	Isotope labeling	Concentration
1	The protein	[U-100% 13C; U-100% 15N]	1.5 mM
2	sodium phosphate	natural abundance	25 mM
3	sodium chloride	natural abundance	150 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	internal	indirect	0.2514495
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	internal	indirect	0.1013291

Referencing offset: -0.2 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	internal	indirect	0.2514495
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	internal	indirect	0.1013291

Referencing offset: -0.2 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	internal	indirect	0.2514495
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	internal	indirect	0.1013291

Referencing offset: 0.12 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	internal	indirect	0.2514495
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	internal	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 12 models in PDB: 2M7L, Strand ID: A Detail

Release date

2013-06-03

Citation

alfa-actinin from parasite Entamoeba histolytica
Persson, C.K., Mayzel, M.L., Karlsson, G.B., Backman, L.
Not known

Related entities 1. alfa-actinin from parasite Entamoeba histolytica, : 1 : 256 entities Detail

Experiments performed 6 experiments Detail

1 2D 1H-15N HSQC/HMQC