BMRB: 19202

NMDA RECEPTOR ANTAGONIST, CONANTOKIN BK-B, NMR, 20 STRUCTURE

Authors

Curtice, K.J., Platt, R.J., Skalicky, J.J., Horvath, M.P., Twede, V.D.

Assembly

CONANTOKIN BK-B

Entity

1. CONANTOKIN BK-B (polymer, Thiol state: not present), 10 monomers, 1156.046 Da Detail

GEXXYSXAIX

Formula weight

1156.046 Da

Source organism

Conus sulcatus

Exptl. method

solution NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 60.0 %, Completeness: 58.9 %, Completeness (bb): 56.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C
All	58.9 % (33 of 56)	61.3 % (19 of 31)	56.0 % (14 of 25)
Backbone	56.7 % (17 of 30)	53.8 % (7 of 13)	58.8 % (10 of 17)
Sidechain	64.5 % (20 of 31)	66.7 % (12 of 18)	61.5 % (8 of 13)
Aromatic	0.0 % (0 of 8)	0.0 % (0 of 4)	0.0 % (0 of 4)
Methyl	100.0 % (6 of 6)	100.0 % (3 of 3)	100.0 % (3 of 3)

1. CONANTOKIN BK-B

GEXXYSXAIX

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Sample

Solvent system trifluoroethanol/water, Pressure 1 atm, Temperature 298 K, pH 6.1, Details 1.1 MM CONANTOKIN BK-B, 10 MM MES, 11 MM CALCIUM CHLORIDE, 90 H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CONANTOKIN BK-B	natural abundance		1.1 mM
2	MES	natural abundance		10 mM
3	Calcium Chloride	natural abundance		11 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2M7R, Strand ID: A Detail

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Release date

2014-06-04

Citation

From molecular phylogeny towards differentiating pharmacology for NMDA receptor subtypes
Platt, R.J., Curtice, K.J., Twede, V.D., Watkins, M., Gruszczyski, P., Bulaj, G., Horvath, M.P., Olivera, B.M.
Toxicon. (2014), 81, 67-79, PubMed 24508768 , DOI 10.1016/j.toxicon.2014.01.016 , Abstract

Experiments performed 2 experiments Detail