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NMDA RECEPTOR ANTAGONIST, CONANTOKIN BK-B, NMR, 20 STRUCTURE
Authors
Curtice, K.J., Platt, R.J., Skalicky, J.J., Horvath, M.P., Twede, V.D.
Assembly
CONANTOKIN BK-B
Entity
1. CONANTOKIN BK-B (polymer, Thiol state: not present), 10 monomers, 1156.046 Da Detail

GEXXYSXAIX


Formula weight
1156.046 Da
Source organism
Conus sulcatus
Exptl. method
solution NMR
Refine. method
SIMULATED ANNEALING
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 60.0 %, Completeness: 58.9 %, Completeness (bb): 56.7 % Detail

Polymer type: polypeptide(L)

Total1H13C
All58.9 % (33 of 56)61.3 % (19 of 31)56.0 % (14 of 25)
Backbone56.7 % (17 of 30)53.8 % (7 of 13)58.8 % (10 of 17)
Sidechain64.5 % (20 of 31)66.7 % (12 of 18)61.5 % (8 of 13)
Aromatic 0.0 % (0 of 8) 0.0 % (0 of 4) 0.0 % (0 of 4)
Methyl100.0 % (6 of 6)100.0 % (3 of 3)100.0 % (3 of 3)

1. CONANTOKIN BK-B

GEXXYSXAIX

Sample

Solvent system trifluoroethanol/water, Pressure 1 atm, Temperature 298 K, pH 6.1, Details 1.1 MM CONANTOKIN BK-B, 10 MM MES, 11 MM CALCIUM CHLORIDE, 90 H2O/10% D2O


#NameIsotope labelingTypeConcentration
1CONANTOKIN BK-Bnatural abundance1.1 mM
2MESnatural abundance10 mM
3Calcium Chloridenatural abundance11 mM
4H2Onatural abundance90 %
5D2Onatural abundance10 %

Protein Blocks Logo
Calculated from 20 models in PDB: 2M7R, Strand ID: A Detail


Release date
2014-06-04
Citation
From molecular phylogeny towards differentiating pharmacology for NMDA receptor subtypes
Platt, R.J., Curtice, K.J., Twede, V.D., Watkins, M., Gruszczyski, P., Bulaj, G., Horvath, M.P., Olivera, B.M.
Toxicon. (2014), 81, 67-79, PubMed 24508768 , DOI 10.1016/j.toxicon.2014.01.016 ,
Experiments performed 2 experiments Detail
nullKeywords TRANSPORT PROTEIN INHIBITOR