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BMRB: 19203

2M7S

NMR structure of RNA recognition motif 2 (RRM2) of Homo sapiens splicing factor, arginine/serine-rich 1

Authors

Dutta, S.K., Serrano, P., Geralt, M., Wuthrich, K.

Assembly

RRM2 of Homo sapiens splicing factor, arginine/serine-rich 1

Entity

1. RRM2 of Homo sapiens splicing factor, arginine/serine-rich 1 (polymer, Thiol state: all free), 90 monomers, 10189.25 Da Detail

GAPRGRYGPP SRRSENRVVV SGLPPSGSWQ DLKDHMREAG DVCYADVYRD GTGVVEFVRK EDMTYAVRKL DNTKFRSHEG ETAYIRVKVD

Formula weight

10189.25 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 86.8 %, Completeness (bb): 82.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.8 % (885 of 1020)	95.1 % (509 of 535)	73.0 % (289 of 396)	97.8 % (87 of 89)
Backbone	82.3 % (436 of 530)	96.2 % (177 of 184)	67.4 % (176 of 261)	97.6 % (83 of 85)
Sidechain	92.8 % (530 of 571)	94.6 % (332 of 351)	89.8 % (194 of 216)	100.0 % (4 of 4)
Aromatic	70.0 % (56 of 80)	87.5 % (35 of 40)	51.3 % (20 of 39)	100.0 % (1 of 1)
Methyl	96.2 % (75 of 78)	100.0 % (39 of 39)	92.3 % (36 of 39)

1. RRM2

GAPRGRYGPP SRRSENRVVV SGLPPSGSWQ DLKDHMREAG DVCYADVYRD GTGVVEFVRK EDMTYAVRKL DNTKFRSHEG ETAYIRVKVD

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	RRM2	[U-99% 13C; U-99% 15N]	1.2 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	sodium azide	natural abundance	5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 2.6 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 2.6 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 4 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2M7S, Strand ID: A Detail

Release date

2013-05-12

Citation

NMR structure of RNA recognition motif 2 (RRM2) of Homo sapiens splicing factor, arginine/serine-rich 1
Dutta, S.K., Serrano, P., Geralt, M., Wuthrich, K.
Not known

Related entities 1. RRM2 of Homo sapiens splicing factor, arginine/serine-rich 1, : 1 : 8 : 3 : 83 entities Detail

Interaction partners 1. RRM2 of Homo sapiens splicing factor, arginine/serine-rich 1, : 106 interactors Detail

More details for 106 interactors identified by 46 citations

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC