BMRB: 19216

Structural and Functional Analysis of Transmembrane Segment IV of the Salt Tolerance Protein Sod2

Authors

Ullah, A., Kemp, G., Lee, B., Alves, C., Young, H., Sykes, B.D., Fliegel, L.

Assembly

sod2 TM IV

Entity

1. sod2 TM IV (polymer, Thiol state: all free), 38 monomers, 3986.804 Da Detail

GSKKKLFPQI NFLGSLLIAG CITSTDPVLS ALIVGKKK

Formula weight

3986.804 Da

Source organism

Schizosaccharomyces pombe

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 64.2 %, Completeness (bb): 94.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	64.2 % (176 of 274)	59.7 % (141 of 236)	92.1 % (35 of 38)
Backbone	94.7 % (108 of 114)	93.6 % (73 of 78)	97.2 % (35 of 36)
Sidechain	42.5 % (68 of 160)	43.0 % (68 of 158)	0.0 % (0 of 2)
Aromatic	0.0 % (0 of 10)	0.0 % (0 of 10)
Methyl	60.7 % (17 of 28)	60.7 % (17 of 28)

1. sod2 TM IV

GSKKKLFPQI NFLGSLLIAG CITSTDPVLS ALIVGKKK

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Sample

Solvent system CDCl3/2-propanol, Pressure 1 atm, Temperature 303.15 K, Details Sample in deuterated chloroform/isopropanol

#	Name	Isotope labeling	Type	Concentration
1	sod2_TM_IV	[U-99% 15N]		0.2 ~ 0.7 mM
2	CDCl3	natural abundance		50 v/v
3	2-propanol	natural abundance		50 v/v

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Protein Blocks Logo

Calculated from 25 models in PDB: 2M7X, Strand ID: A Detail

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Release date

2013-06-03

Citation

Structural and functional analysis of transmembrane segment IV of the salt tolerance protein Sod2
Ullah, A., Kemp, G., Lee, B., Alves, C., Young, H., Sykes, B.D., Fliegel, L.
J. Biol. Chem. (2013), 288, 24609-24624, PubMed 23836910 , DOI 10.1074/jbc.M113.483065 , Abstract

Related entities 1. sod2 TM IV, : 9 entities Detail

Experiments performed 4 experiments Detail