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BMRB: 19217

2M7Z

SmTSP2EC2

Authors

Mulvenna, J., Jia, X.

Assembly

SmTSP2EC2

Entity

1. SmTSP2EC2 (polymer, Thiol state: all disulfide bound), 81 monomers, 9071.184 Da Detail

GSNEKPKVKK HITSALKKLV DKYRNDEHVR KVFDEIQQKL HCCGADSPKD YGENPPTSCS KDGVQFTEGC IKKVSDLSKA H

Formula weight

9071.184 Da

Source organism

Schistosoma mansoni

Exptl. method

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 89.6 %, Completeness (bb): 83.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.6 % (849 of 948)	96.4 % (485 of 503)	77.9 % (282 of 362)	98.8 % (82 of 83)
Backbone	83.7 % (400 of 478)	97.5 % (159 of 163)	69.3 % (165 of 238)	98.7 % (76 of 77)
Sidechain	96.2 % (525 of 546)	95.9 % (326 of 340)	96.5 % (193 of 200)	100.0 % (6 of 6)
Aromatic	76.9 % (40 of 52)	76.9 % (20 of 26)	76.9 % (20 of 26)
Methyl	96.9 % (62 of 64)	96.9 % (31 of 32)	96.9 % (31 of 32)

1. SmTSP2EC2

GSNEKPKVKK HITSALKKLV DKYRNDEHVR KVFDEIQQKL HCCGADSPKD YGENPPTSCS KDGVQFTEGC IKKVSDLSKA H

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.2

#	Name	Isotope labeling	Type	Concentration
1	SmTSP2EC2	[U-100% 13C; U-100% 15N]		1.4 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

LACS Plot; CA

Referencing offset: -0.33 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: -0.33 ppm, Outliers: 1 Detail

LACS Plot; HA

Referencing offset: 0.01 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2M7Z, Strand ID: A Detail

Release date

2014-01-20

Citation

Solution structure, membrane interactions, and protein binding partners of the tetraspanin Sm-TSP-2, a vaccine antigen from the human blood fluke Schistosoma mansoni
Jia, X., Schulte, L., Loukas, A., Pickering, D., Pearson, M., Mobli, M., Jones, A., Rosengren, K.J., Daly, N.L., Gobert, G.N., Jones, M.K., Craik, D.J., Mulvenna, J.
J. Biol. Chem. (2014), 289, 7151-7163, PubMed 24429291 , DOI 10.1074/jbc.M113.531558 , Abstract

Related entities 1. SmTSP2EC2, : 1 : 30 entities Detail

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.2

#	Name	Isotope labeling	Type	Concentration
1	SmTSP2EC2	[U-100% 13C; U-100% 15N]		1.4 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

2 2D 1H-13C HSQC aliphatic

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.2

#	Name	Isotope labeling	Type	Concentration
1	SmTSP2EC2	[U-100% 13C; U-100% 15N]		1.4 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

3 2D 1H-13C HSQC aromatic

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.2

#	Name	Isotope labeling	Type	Concentration
1	SmTSP2EC2	[U-100% 13C; U-100% 15N]		1.4 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

4 3D CBCA(CO)NH

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.2

#	Name	Isotope labeling	Type	Concentration
1	SmTSP2EC2	[U-100% 13C; U-100% 15N]		1.4 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

5 3D HNCA

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.2

#	Name	Isotope labeling	Type	Concentration
1	SmTSP2EC2	[U-100% 13C; U-100% 15N]		1.4 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

6 3D HNCACB

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.2

#	Name	Isotope labeling	Type	Concentration
1	SmTSP2EC2	[U-100% 13C; U-100% 15N]		1.4 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

7 3D HNCO

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.2

#	Name	Isotope labeling	Type	Concentration
1	SmTSP2EC2	[U-100% 13C; U-100% 15N]		1.4 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

8 3D HBHA(CO)NH

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.2

#	Name	Isotope labeling	Type	Concentration
1	SmTSP2EC2	[U-100% 13C; U-100% 15N]		1.4 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

9 3D 1H-15N NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.2

#	Name	Isotope labeling	Type	Concentration
1	SmTSP2EC2	[U-100% 13C; U-100% 15N]		1.4 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

10 2D 1H-13C HSQC aliphatic

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.2

#	Name	Isotope labeling	Type	Concentration
1	SmTSP2EC2	[U-100% 13C; U-100% 15N]		1.4 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

11 3D 1H-13C NOESY aromatic

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.2

#	Name	Isotope labeling	Type	Concentration
1	SmTSP2EC2	[U-100% 13C; U-100% 15N]		1.4 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

12 3D HN(CA)CO

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.2

#	Name	Isotope labeling	Type	Concentration
1	SmTSP2EC2	[U-100% 13C; U-100% 15N]		1.4 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

13 4D HCC(CO)NH-TOCSY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.2

#	Name	Isotope labeling	Type	Concentration
1	SmTSP2EC2	[U-100% 13C; U-100% 15N]		1.4 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

14 2D (H)CB-(CGCCTOCSY)-Har

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.2

#	Name	Isotope labeling	Type	Concentration
1	SmTSP2EC2	[U-100% 13C; U-100% 15N]		1.4 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

15 Tyr-selective 2D (H)CB-(CGCCTOCSY)-Har

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.2

#	Name	Isotope labeling	Type	Concentration
1	SmTSP2EC2	[U-100% 13C; U-100% 15N]		1.4 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

16 Phe-selective 2D (H)CB-(CGCCTOCSY)-Har

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.2

#	Name	Isotope labeling	Type	Concentration
1	SmTSP2EC2	[U-100% 13C; U-100% 15N]		1.4 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

nullKeywords Tetraspanin