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BMRB: 19219

2MR3

1H, 13C, and 15N Chemical Shift Assignments for a proteasome related subunit

Authors

Wu, Y., Hu, Y., Jin, C.

Assembly

Yeast Rpn9

Entity

1. Yeast Rpn9 (polymer, Thiol state: all free), 401 monomers, 46846.99 Da Detail

MFNNHEIDTI LSTLRMEADP SLHPLFEQFE KFYEEKLWFQ LSESLTKFFD DAKSTPLRLR LYDNFVSKFY DKINQLSVVK YLLASLKDSK DFDESLKYLD DLKAQFQELD SKKQRNNGSK DHGDGILLID SEIARTYLLK NDLVKARDLL DDLEKTLDKK DSIPLRITNS FYSTNSQYFK FKNDFNSFYY TSLLYLSTLE PSTSITLAER QQLAYDLSIS ALLGDKIYNF GELLHHPIME TIVNDSNYDW LFQLLNALTV GDFDKFDSLI KVQISKIPIL AQHESFLRQK ICLMTLIETV FVKNIRMLSF EDISKATHLP KDNVEHLVMR AISLGLLKGS IDQVNELVTI SWVQPRIISG DQITKMKDRL VEWNDQVEKL GKKMEARGQS IWVLEHHHHH H

Formula weight

46846.99 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.8 %, Completeness: 80.8 %, Completeness (bb): 95.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	80.8 % (3991 of 4942)	77.4 % (1991 of 2572)	82.2 % (1592 of 1936)	94.0 % (408 of 434)
Backbone	95.1 % (2272 of 2388)	94.7 % (761 of 804)	94.9 % (1131 of 1192)	96.9 % (380 of 392)
Sidechain	71.3 % (2098 of 2944)	69.6 % (1230 of 1768)	74.1 % (840 of 1134)	66.7 % (28 of 42)
Aromatic	26.4 % (121 of 458)	30.1 % (69 of 229)	21.0 % (47 of 224)	100.0 % (5 of 5)
Methyl	93.1 % (445 of 478)	93.3 % (223 of 239)	92.9 % (222 of 239)

1. a proteasome subunit

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.7

#	Name	Isotope labeling	Concentration
1	protein	[U-100% 13C; U-100% 15N]	0.7 mM
2	DTT	natural abundance	10 mM
3	MES	natural abundance	50 mM
4	DSS	natural abundance	0.03 %
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.7

#	Name	Isotope labeling	Concentration
7	protein	[U-13C; U-15N; U-2H]	0.7 mM
8	DTT	natural abundance	10 mM
9	MES	natural abundance	50 mM
10	DSS	natural abundance	0.03 %
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MR3, Strand ID: A Detail

Release date

2015-02-01

Citation

Solution structure of yeast Rpn9: insights into proteasome lid assembly
Wu, Y., Hu, Y., Jin, C.
J. Biol. Chem. (2015), 290, 6878-6889, PubMed 25631053 , DOI 10.1074/jbc.M114.626762 , Abstract

Related entities 1. Yeast Rpn9, : 1 : 29 : 86 entities Detail

Interaction partners 1. Yeast Rpn9, : 64 interactors Detail

More details for 64 interactors identified by 10 citations

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC