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BMRB: 19220

2MQW

solution structure of a proteasome related subunit N terminal domain

Authors

Wu, Y., Hu, Y., Jin, C.

Assembly

proteasome related subunit N terminal domain

Entity

1. proteasome related subunit N terminal domain (polymer, Thiol state: not present), 163 monomers, 19177.46 Da Detail

GSHMFNNHEI DTILSTLRME ADPSLHPLFE QFEKFYEEKL WFQLSESLTK FFDDAKSTPL RLRLYDNFVS KFYDKINQLS VVKYLLASLK DSKDFDESLK YLDDLKAQFQ ELDSKKQRNN GSKDHGDGIL LIDSEIARTY LLKNDLVKAR DLLDDLEKTL DKK

Formula weight

19177.46 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.5 %, Completeness: 85.8 %, Completeness (bb): 94.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.8 % (1731 of 2017)	83.9 % (887 of 1057)	85.9 % (675 of 786)	97.1 % (169 of 174)
Backbone	94.0 % (914 of 972)	94.8 % (310 of 327)	92.6 % (449 of 485)	96.9 % (155 of 160)
Sidechain	80.8 % (973 of 1204)	79.0 % (577 of 730)	83.0 % (382 of 460)	100.0 % (14 of 14)
Aromatic	44.6 % (83 of 186)	47.3 % (44 of 93)	41.3 % (38 of 92)	100.0 % (1 of 1)
Methyl	94.2 % (162 of 172)	94.2 % (81 of 86)	94.2 % (81 of 86)

1. entity

GSHMFNNHEI DTILSTLRME ADPSLHPLFE QFEKFYEEKL WFQLSESLTK FFDDAKSTPL RLRLYDNFVS KFYDKINQLS VVKYLLASLK DSKDFDESLK YLDDLKAQFQ ELDSKKQRNN GSKDHGDGIL LIDSEIARTY LLKNDLVKAR DLLDDLEKTL DKK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	protein	[U-100% 13C; U-100% 15N]	0.5 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	100 mM
4	DSS	natural abundance	0.03 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.29 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MQW, Strand ID: A Detail

Release date

2015-02-01

Citation

Solution structure of yeast Rpn9: insights into proteasome lid assembly
Wu, Y., Hu, Y., Jin, C.
J. Biol. Chem. (2015), 290, 6878-6889, PubMed 25631053 , DOI 10.1074/jbc.M114.626762 , Abstract

Related entities 1. proteasome related subunit N terminal domain, : 1 : 27 : 58 entities Detail

Interaction partners 1. proteasome related subunit N terminal domain, : 64 interactors Detail

More details for 64 interactors identified by 10 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC