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BMRB: 19221

2MRI

solution structure of a proteasome related subunit C terminal domain

Authors

Wu, Y., Hu, Y., Jin, C.

Assembly

proteasome related subunit C terminal domain

Entity

1. proteasome related subunit C terminal domain (polymer, Thiol state: all free), 176 monomers, 20227.17 Da Detail

FKNDFNSFYY TSLLYLSTLE PSTSITLAER QQLAYDLSIS ALLGDKIYNF GELLHHPIME TIVNDSNYDW LFQLLNALTV GDFDKFDSLI KVQISKIPIL AQHESFLRQK ICLMTLIETV FVKNIRMLSF EDISKATHLP KDNVEHLVMR AISLGLLKGS IDQVNELVTI SWVQPR

Formula weight

20227.17 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.1 %, Completeness (bb): 98.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.1 % (1979 of 2148)	90.5 % (1002 of 1107)	92.6 % (788 of 851)	99.5 % (189 of 190)
Backbone	98.5 % (1030 of 1046)	97.7 % (344 of 352)	98.7 % (516 of 523)	99.4 % (170 of 171)
Sidechain	87.4 % (1112 of 1273)	86.8 % (655 of 755)	88.0 % (439 of 499)	94.7 % (18 of 19)
Aromatic	46.9 % (90 of 192)	46.9 % (45 of 96)	45.7 % (43 of 94)	100.0 % (2 of 2)
Methyl	95.1 % (234 of 246)	95.1 % (117 of 123)	95.1 % (117 of 123)

1. entity

FKNDFNSFYY TSLLYLSTLE PSTSITLAER QQLAYDLSIS ALLGDKIYNF GELLHHPIME TIVNDSNYDW LFQLLNALTV GDFDKFDSLI KVQISKIPIL AQHESFLRQK ICLMTLIETV FVKNIRMLSF EDISKATHLP KDNVEHLVMR AISLGLLKGS IDQVNELVTI SWVQPR

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	protein	[U-100% 13C; U-100% 15N]	0.5 mM
2	TRIS	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	DTT	natural abundance	10 mM
5	DSS	natural abundance	0.03 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.25 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.25 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MRI, Strand ID: A Detail

Release date

2015-02-01

Citation

Solution structure of yeast Rpn9: insights into proteasome lid assembly
Wu, Y., Hu, Y., Jin, C.
J. Biol. Chem. (2015), 290, 6878-6889, PubMed 25631053 , DOI 10.1074/jbc.M114.626762 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 19219 released on 2015-02-01
    Title 1H, 13C, and 15N Chemical Shift Assignments for a proteasome related subunit
  BMRB: 19220 released on 2015-02-01
    Title solution structure of a proteasome related subunit N terminal domain

Related entities 1. proteasome related subunit C terminal domain, : 1 : 16 : 59 entities Detail

Interaction partners 1. proteasome related subunit C terminal domain, : 64 interactors Detail

More details for 64 interactors identified by 10 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC