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BMRB: 19229

2M85

PHD Domain from Human SHPRH

Authors

Machado, L.ESF., Pustovalova, Y., Pozhidaeva, A., Almeida, F.CL., Bezsonova, I., Korzhnev, D.M.

Assembly

PHD Domain from Human SHPRH

Entity

1. SHPRH (polymer), 73 monomers, 8575.795 Da Detail

GSPNSRVDFN TSDYRFECIC GELDQIDRKP RVQCLKCHLW QHAKCVNYDE KNLKIKPFYC PHCLVAMEPV STR

2. ZINC ION (non-polymer, Thiol state: not present), 65.409 × 2 Da

Total weight

8706.613 Da

Max. entity weight

8575.795 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.8 %, Completeness: 89.6 %, Completeness (bb): 91.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.6 % (786 of 877)	90.3 % (418 of 463)	87.9 % (297 of 338)	93.4 % (71 of 76)
Backbone	91.4 % (391 of 428)	91.6 % (131 of 143)	90.8 % (197 of 217)	92.6 % (63 of 68)
Sidechain	88.8 % (462 of 520)	90.0 % (288 of 320)	86.5 % (166 of 192)	100.0 % (8 of 8)
Aromatic	73.1 % (57 of 78)	84.6 % (33 of 39)	60.5 % (23 of 38)	100.0 % (1 of 1)
Methyl	93.3 % (56 of 60)	93.3 % (28 of 30)	93.3 % (28 of 30)

1. SHPRH

GSPNSRVDFN TSDYRFECIC GELDQIDRKP RVQCLKCHLW QHAKCVNYDE KNLKIKPFYC PHCLVAMEPV STR

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	HEPES	[U-100% 13C; U-100% 15N]	50 mM
2	sodium chloride	natural abundance	150 mM
3	ZINC ION	natural abundance	0.05 mM
4	DTT	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291
³¹P	DSS	methyl protons	na	indirect	0.4048086

Referencing offset: -0.07 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291
³¹P	DSS	methyl protons	na	indirect	0.4048086

Referencing offset: -0.07 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291
³¹P	DSS	methyl protons	na	indirect	0.4048086

Referencing offset: 0.13 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291
³¹P	DSS	methyl protons	na	indirect	0.4048086

Referencing offset: 0.52 ppm, Outliers: 5 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2M85, Strand ID: A Detail

Release date

2013-08-11

Citation

PHD domain from human SHPRH
Machado, L.E.F., Pustovalova, Y., Kile, A.C., Pozhidaeva, A., Cimprich, K.A., Almeida, F.C.L., Bezsonova, I., Korzhnev, D.M.
J. Biomol. NMR (2013), 56, 393-399, PubMed 23907177 , DOI 10.1007/s10858-013-9758-2 , Abstract

Related entities 1. SHPRH, : 1 : 32 entities Detail

Interaction partners 1. SHPRH, : 10 interactors Detail

More details for 10 interactors identified by 5 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC