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Found 1 Document (0.789 seconds)

BMRB: 19231

2M87

Structural Basis of DNA Recognition by the Effector Domain of Klebsiella pneumoniae PmrA

Authors

Wang, I., Lou, Y., Chen, C.

Assembly

PmrA

Entity

1. PmrA (polymer, Thiol state: not present), 103 monomers, 11838.32 Da Detail

NQGDNEISVG NLRLNVTRRL VWLGETALDL TPKEYALLSR LMMKAGSPVH REILYNDIYS WDNEPATNTL EVHIHNLREK IGKSRIRTVR GFGYMLANNI DTE

Formula weight

11838.32 Da

Source organism

Klebsiella pneumoniae

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 96.5 %, Completeness (bb): 97.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.5 % (1172 of 1214)	95.4 % (603 of 632)	97.7 % (458 of 469)	98.2 % (111 of 113)
Backbone	97.9 % (599 of 612)	97.6 % (205 of 210)	98.0 % (296 of 302)	98.0 % (98 of 100)
Sidechain	95.7 % (668 of 698)	94.3 % (398 of 422)	97.7 % (257 of 263)	100.0 % (13 of 13)
Aromatic	100.0 % (78 of 78)	100.0 % (39 of 39)	100.0 % (37 of 37)	100.0 % (2 of 2)
Methyl	98.4 % (126 of 128)	96.9 % (62 of 64)	100.0 % (64 of 64)

1. entity

NQGDNEISVG NLRLNVTRRL VWLGETALDL TPKEYALLSR LMMKAGSPVH REILYNDIYS WDNEPATNTL EVHIHNLREK IGKSRIRTVR GFGYMLANNI DTE

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	PmrAc	[U-99% 13C; U-99% 15N]	0.3 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
4	PmrAc	[U-99% 13C; U-99% 15N]		0.3 mM
5	D2O	natural abundance		100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	nitrogen	na	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	nitrogen	na	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	nitrogen	na	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	nitrogen	na	indirect	0.1013291

Referencing offset: 0.18 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 2M87, Strand ID: A Detail

Release date

2014-01-20

Citation

Solution structure and tandem DNA recognition of the C-terminal effector domain of PmrA from Klebsiella pneumoniae
Lou, Y., Wang, I., Rajasekaran, M., Kao, Y., Ho, M., Hsu, S., Chou, S., Wu, S., Chen, C.
Nucleic Acids Res. (2014), 42, 4080-4093, PubMed 24371275 , DOI 10.1093/nar/gkt1345 , Abstract

Related entities 1. PmrA, : 1 : 2 : 149 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	PmrAc	[U-99% 13C; U-99% 15N]	0.3 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

2 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	PmrAc	[U-99% 13C; U-99% 15N]	0.3 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

3 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	PmrAc	[U-99% 13C; U-99% 15N]	0.3 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

4 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	PmrAc	[U-99% 13C; U-99% 15N]	0.3 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

5 3D HBHA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	PmrAc	[U-99% 13C; U-99% 15N]	0.3 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

6 2D 1H-13C HSQC aliphatic

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
4	PmrAc	[U-99% 13C; U-99% 15N]		0.3 mM
5	D2O	natural abundance		100 %

7 3D HCCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
4	PmrAc	[U-99% 13C; U-99% 15N]		0.3 mM
5	D2O	natural abundance		100 %

8 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	PmrAc	[U-99% 13C; U-99% 15N]	0.3 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

9 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
4	PmrAc	[U-99% 13C; U-99% 15N]		0.3 mM
5	D2O	natural abundance		100 %

10 3D 1H-13C NOESY aromatic

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
4	PmrAc	[U-99% 13C; U-99% 15N]		0.3 mM
5	D2O	natural abundance		100 %

nullKeywords DNA-binding, effector domain, NMR, PmrA, two-component signaling system

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Found 1 Document (0.789 seconds)

BMRB: 19231

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. PmrA, : 1 : 2 : 149 entities Detail

Experiments performed 10 experiments Detail

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