NMR structure of a two-domain RNA-binding fragment of Nrd1
PDELNVSNNP HYRPKPVSYD STLPPDHIKV YSRTLFIGGV PLNMKEWDLA NVLKPFAEVQ SVILNNSRKH AFVKVYSRHE AENVLQNFNK DGALPLRTRW GVGFGPRDCC DYQHGYSIIP MHRLTDADKK WSVSAQWGGT SGQPLVTGIV FEEPDIIVGE GVSSKAISQK MPTDSGRNGP RSGKP
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 24.1 % (519 of 2151) | 23.6 % (266 of 1126) | 14.6 % (122 of 835) | 68.9 % (131 of 190) |
Backbone | 28.4 % (306 of 1078) | 37.8 % (140 of 370) | 7.2 % (39 of 539) | 75.1 % (127 of 169) |
Sidechain | 18.0 % (223 of 1242) | 16.7 % (126 of 756) | 20.0 % (93 of 465) | 19.0 % (4 of 21) |
Aromatic | 8.9 % (16 of 180) | 8.9 % (8 of 90) | 4.7 % (4 of 86) | 100.0 % (4 of 4) |
Methyl | 63.5 % (113 of 178) | 64.0 % (57 of 89) | 62.9 % (56 of 89) |
1. entity
PDELNVSNNP HYRPKPVSYD STLPPDHIKV YSRTLFIGGV PLNMKEWDLA NVLKPFAEVQ SVILNNSRKH AFVKVYSRHE AENVLQNFNK DGALPLRTRW GVGFGPRDCC DYQHGYSIIP MHRLTDADKK WSVSAQWGGT SGQPLVTGIV FEEPDIIVGE GVSSKAISQK MPTDSGRNGP RSGKPSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity | [U-13C; U-15N] | 0.4 mM | |
2 | sodium phosphate | natural abundance | 50 mM | |
3 | sodium chloride | natural abundance | 300 mM | |
4 | beta-mercaptoethanol | natural abundance | 10 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | entity | [U-100% 13C; U-100% 15N; U-80% 2H] | 0.4 mM | |
6 | sodium phosphate | natural abundance | 50 mM | |
7 | sodium chloride | natural abundance | 300 mM | |
8 | beta-mercaptoethanol | natural abundance | 10 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 8, Details selectively protonated I, L, V amino acids
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | entity | [U-13C; U-15N; U-2H] | 0.4 mM | |
10 | sodium phosphate | natural abundance | 50 mM | |
11 | sodium chloride | natural abundance | 300 mM | |
12 | beta-mercaptoethanol | natural abundance | 10 mM |