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BMRB: 19248

2M8D

Structure of SRSF1 RRM2 in complex with the RNA 5'-UGAAGGAC-3'

Authors

Clery, A., Sinha, R., Anczukow, O., Corrionero, A., Moursy, A., Daubner, G., Valcarcel, J., Krainer, A.R., Allain, F.HT.

Assembly

SRSF1 RRM2 in complex with the RNA 5'-UGAAGGAC-3'

Entity

1. RNA 5'-UGAAGGAC-3 (polymer, Thiol state: not present), 8 monomers, 2652.597 Da Detail

UGAAGGAC

2. SRSF1 RRM2 (polymer), 91 monomers, 10320.45 Da Detail

MAPRGRYGPP SRRSENRVVV SGLPPSGSWQ DLKDHMREAG DVCYADVYRD GTGVVEFVRK EDMTYAVRKL DNTKFRSHEG ETAYIRVKVD G

Total weight

12973.047 Da

Max. entity weight

10320.45 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.0 %, Completeness: 80.4 %, Completeness (bb): 76.8 % Detail

Polymer type: polyribonucleotide polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	80.4 % (930 of 1157)	92.6 % (564 of 609)	61.2 % (278 of 454)	93.6 % (88 of 94)
Backbone	76.8 % (479 of 624)	97.4 % (228 of 234)	54.6 % (166 of 304)	98.8 % (85 of 86)
Sidechain	85.9 % (528 of 615)	89.6 % (336 of 375)	81.5 % (189 of 232)	37.5 % (3 of 8)
Aromatic	69.3 % (79 of 114)	93.0 % (53 of 57)	48.1 % (25 of 52)	20.0 % (1 of 5)
Methyl	94.9 % (74 of 78)	97.4 % (38 of 39)	92.3 % (36 of 39)

1. RNA 5'-UGAAGGAC-3

UGAAGGAC

2. SRSF1 RRM2

MAPRGRYGPP SRRSENRVVV SGLPPSGSWQ DLKDHMREAG DVCYADVYRD GTGVVEFVRK EDMTYAVRKL DNTKFRSHEG ETAYIRVKVD G

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	SRSF1_RRM2	[U-100% 15N]	0.8 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 5.5

#	Name	Isotope labeling	Concentration
4	SRSF1_RRM2	[U-100% 13C; U-100% 15N]	0.8 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	internal	direct	1.0
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	internal	direct	1.0

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	internal	direct	1.0
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	internal	direct	1.0

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	internal	direct	1.0
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	internal	direct	1.0

Referencing offset: -0.19 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 16 models in PDB: 2M8D, Strand ID: B Detail

Release date

2013-07-07

Citation

Isolated pseudo-RNA-recognition motifs of SR proteins can regulate splicing using a noncanonical mode of RNA recognition
Clery, A., Sinha, R., Anczukow, O., Corrionero, A., Moursy, A., Daubner, G.M., Valcarcel, J., Krainer, A.R., Allain, F.
Proc. Natl. Acad. Sci. U. S. A. (2013), 110, E2802-E2811, PubMed 23836656 , DOI 10.1073/pnas.1303445110 , Abstract

Related entities 2. SRSF1 RRM2, : 1 : 1 : 10 : 75 entities Detail

Interaction partners 2. SRSF1 RRM2, : 106 interactors Detail

More details for 106 interactors identified by 46 citations

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC