NMR assignment and structure of a peptide derived from the membrane proximal external region of HIV-1 gp41 in DPC
Polymer type: polypeptide(L)
Total | 1H | 13C | |
---|---|---|---|
All | 61.0 % (214 of 351) | 98.5 % (198 of 201) | 10.7 % (16 of 150) |
Backbone | 44.3 % (62 of 140) | 96.4 % (54 of 56) | 9.5 % (8 of 84) |
Sidechain | 64.9 % (155 of 239) | 99.3 % (144 of 145) | 11.7 % (11 of 94) |
Aromatic | 52.1 % (38 of 73) | 97.4 % (38 of 39) | 0.0 % (0 of 34) |
Methyl | 68.8 % (22 of 32) | 100.0 % (16 of 16) | 37.5 % (6 of 16) |
1. MPERp
NEQELLELDK WASLWNWFNI TNWLWYIKSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | MPERp | natural abundance | 0.5 mM | |
2 | H2O | natural abundance | 90 % | |
3 | D2O | [U-100% 2H] | 10 % | |
4 | DPC | [U-99% 2H] | 20 mM | |
5 | HEPES | natural abundance | 2 mM | |
6 | DSS | natural abundance | 0.1 mM |