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BMRB: 19264

2M8P

The structure of the W184AM185A mutant of the HIV-1 capsid protein

Authors

Deshmukh, L., Schwieters, C.D., Grishaev, A., Clore, G., Ghirlando, R.

Assembly

W184AM185A mutant of the HIV-1 capsid protein

Entity

1. W184AM185A mutant of the HIV-1 capsid protein (polymer), 231 monomers, 25426.91 Da Detail

PIVQNLQGQM VHQAISPRTL NAWVKVVEEK AFSPEVIPMF SALSEGATPQ DLNTMLNTVG GHQAAMQMLK ETINEEAAEW DRLHPVHAGP IAPGQMREPR GSDIAGTTST LQEQIGWMTH NPPIPVGEIY KRWIILGLNK IVRMYSPTSI LDIRQGPKEP FRDYVDRFYK TLRAEQASQE VKNAATETLL VQNANPDCKT ILKALGPGAT LEEMMTACQG VGGPGHKARV L

Formula weight

25426.91 Da

Source organism

Human immunodeficiency virus 1

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.3 %, Completeness: 44.5 %, Completeness (bb): 80.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	44.5 % (1184 of 2663)	22.5 % (313 of 1390)	64.3 % (663 of 1031)	86.0 % (208 of 242)
Backbone	80.2 % (1083 of 1350)	50.6 % (234 of 462)	95.4 % (644 of 675)	96.2 % (205 of 213)
Sidechain	19.7 % (300 of 1526)	8.4 % (78 of 928)	38.7 % (220 of 569)	6.9 % (2 of 29)
Aromatic	0.0 % (0 of 144)	0.0 % (0 of 72)	0.0 % (0 of 68)	0.0 % (0 of 4)
Methyl	16.9 % (45 of 266)	12.0 % (16 of 133)	21.8 % (29 of 133)

1. entity

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-13C; U-15N; U-2H]	0.5 mM
2	EDTA	natural abundance	1 mM
3	sodium chloride	natural abundance	50 mM
4	DTT	natural abundance	1 mM
5	sodium phosphate	natural abundance	20 mM
6	H2O	natural abundance	95 %
7	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	internal	indirect	1.0
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	internal	indirect	1.0

Referencing offset: 0.71 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	internal	indirect	1.0
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	internal	indirect	1.0

Referencing offset: 0.71 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	internal	indirect	1.0
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	internal	indirect	1.0

Referencing offset: -0.14 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 100 models in PDB: 2M8P, Strand ID: A Detail

Release date

2013-11-17

Citation

Structure and dynamics of full-length HIV-1 capsid protein in solution
Deshmukh, L., Schwieters, C.D., Grishaev, A., Ghirlando, R., Baber, J.L., Clore, G.
J. Am. Chem. Soc. (2013), 135, 16133-16147, PubMed 24066695 , DOI 10.1021/ja406246z , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 19261 released on 2013-12-08
Title HIV capsid dimer structure

Related entities 1. W184AM185A mutant of the HIV-1 capsid protein, : 1 : 1 : 101 : 122 entities Detail

Interaction partners 1. W184AM185A mutant of the HIV-1 capsid protein, : 4 interactors Detail

More details for 4 interactors identified by 3 citations

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC