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BMRB: 19282

2MTI

NMR structure of the lymphocyte receptor NKR-P1A

Authors

Chmelik, J., Rozbesky, D., Pospisilova, E., Novak, P.

Assembly

lymphocyte receptor NKR-P1A

Entity

1. lymphocyte receptor NKR-P1A (polymer), 139 monomers, 15991.63 Da Detail

SAKLECPQDW LSHRDKCFHV SQVSNTWEEG LVDCDGKGAT LMLIQDQEEL RFLLDSIKEK YNSFWIGLRY TLPDMNWKWI NGSTLNSDVL KITGDTENDS CAAISGDKVT FESCNSDNRW ICQKELYHET LSNYVGYGH

Formula weight

15991.63 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.6 %, Completeness (bb): 98.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	98.6 % (1602 of 1624)	98.1 % (824 of 840)	99.4 % (623 of 627)	98.7 % (155 of 157)
Backbone	98.7 % (819 of 830)	97.5 % (278 of 285)	99.5 % (406 of 408)	98.5 % (135 of 137)
Sidechain	98.8 % (913 of 924)	98.4 % (546 of 555)	99.4 % (347 of 349)	100.0 % (20 of 20)
Aromatic	97.6 % (164 of 168)	97.6 % (82 of 84)	97.4 % (76 of 78)	100.0 % (6 of 6)
Methyl	100.0 % (132 of 132)	100.0 % (66 of 66)	100.0 % (66 of 66)

1. NKR-P1A

SAKLECPQDW LSHRDKCFHV SQVSNTWEEG LVDCDGKGAT LMLIQDQEEL RFLLDSIKEK YNSFWIGLRY TLPDMNWKWI NGSTLNSDVL KITGDTENDS CAAISGDKVT FESCNSDNRW ICQKELYHET LSNYVGYGH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	NKR-P1A	[U-100% 13C; U-100% 15N]	0.5 mM
2	PIPES	natural abundance	15 mM
3	sodium chloride	natural abundance	50 mM
4	sodium azide	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.31 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2MTI, Strand ID: A Detail

Release date

2014-06-08

Citation

NMR structure of the lymphocyte receptor NKR-P1A reveals a different conformation of the conserved loop compared to crystal structure
Rozbesky, D., Pospisilova, E., Novak, P., Chmelik, J.
Not known

Related entities 1. lymphocyte receptor NKR-P1A, : 1 : 2 : 3 : 286 entities Detail

Experiments performed 17 experiments Detail

1 2D 1H-15N HSQC