BMRB: 19285

Optimized Ratiometric Calcium Sensors For Functional In Vivo Imaging of Neurons and T-Lymphocytes

Authors

Russo, L., Becker, S., Griesinger, C.

Assembly

Calcium Sensor

Entity

1. Calcium Sensor (polymer), 69 monomers, 8059.586 Da Detail

SEEELSECFR IFDKDGNGFI DREEFGDIIR LTGEQLTDED VDEIFGDSDT DKNGRIDFDE FLKMMENVQ

Formula weight

8059.586 Da

Source organism

Opsanus tau

Exptl. method

solution NMR

Refine. method

MFR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 53.1 %, Completeness (bb): 99.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	53.1 % (424 of 798)	36.7 % (153 of 417)	65.8 % (202 of 307)	93.2 % (69 of 74)
Backbone	99.3 % (411 of 414)	99.3 % (143 of 144)	99.0 % (199 of 201)	100.0 % (69 of 69)
Sidechain	16.8 % (75 of 447)	3.7 % (10 of 273)	38.5 % (65 of 169)	0.0 % (0 of 5)
Aromatic	0.0 % (0 of 70)	0.0 % (0 of 35)	0.0 % (0 of 35)
Methyl	0.0 % (0 of 54)	0.0 % (0 of 27)	0.0 % (0 of 27)

1. entity

SEEELSECFR IFDKDGNGFI DREEFGDIIR LTGEQLTDED VDEIFGDSDT DKNGRIDFDE FLKMMENVQ

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	Calcium sensor	[U-13C; U-15N]
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %
4	TRIS	natural abundance		1 mM

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LACS Plot; CA

Referencing offset: 1.0 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 1.0 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.42 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2M97, Strand ID: A Detail

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Release date

2014-01-01

Citation

Optimized ratiometric calcium sensors for functional in vivo imaging of neurons and T lymphocytes
Thestrup, T., Litzlbauer, J., Bartholomaus, I., Mues, M., Russo, L., Dana, H., Kovalchuk, Y., Liang, Y., Kalamakis, G., Laukat, Y., Becker, S., Witte, G., Geiger, A., Allen, T., Rome, L.C., Chen, T., Kim, D.S., Garaschuk, O., Griesinger, C., Griesbeck, O.
Nat. Methods (2014), 11, 175-182, PubMed 24390440 , DOI 10.1038/nmeth.2773 , Abstract

Related entities 1. Calcium Sensor, : 1 : 554 entities Detail

Experiments performed 6 experiments Detail