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Found 1 Document (2.216 seconds)

BMRB: 19298

2M9K

RBPMS2-Nter

Authors

YANG, Y.

Assembly

RBPMS2-Nter

Entity

1. RBPMS2-Nter (polymer, Thiol state: not present), 93 monomers, 10486.04 × 2 Da Detail

MEEEVRTLFV SGLPVDIKPR ELYLLFRPFK GYEGSLIKLT ARQPVGFVIF DSRAGAEAAK NALNGIRFDP ENPQTLRLEF AKANTKMAKS KLE

Total weight

20972.08 Da

Max. entity weight

10486.04 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 52.3 %, Completeness (bb): 96.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	52.3 % (588 of 1124)	38.2 % (226 of 591)	62.5 % (275 of 440)	93.5 % (87 of 93)
Backbone	96.0 % (524 of 546)	97.8 % (182 of 186)	93.8 % (256 of 273)	98.9 % (86 of 87)
Sidechain	21.5 % (143 of 665)	10.9 % (44 of 405)	38.6 % (98 of 254)	16.7 % (1 of 6)
Aromatic	11.6 % (10 of 86)	14.0 % (6 of 43)	9.3 % (4 of 43)
Methyl	23.6 % (25 of 106)	17.0 % (9 of 53)	30.2 % (16 of 53)

1. entity

MEEEVRTLFV SGLPVDIKPR ELYLLFRPFK GYEGSLIKLT ARQPVGFVIF DSRAGAEAAK NALNGIRFDP ENPQTLRLEF AKANTKMAKS KLE

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 305 K, pH 6.3

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 15N]	0.4-0.8 mM
2	entity	[U-100% 13C; U-100% 15N]	0.7 mM
3	H2O	natural abundance	95 %
4	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	118.0 ppm	external	indirect	1.0
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	42.6 ppm	external	indirect	1.0

Referencing offset: 0.07 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	118.0 ppm	external	indirect	1.0
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	42.6 ppm	external	indirect	1.0

Referencing offset: 0.07 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	118.0 ppm	external	indirect	1.0
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	42.6 ppm	external	indirect	1.0

Referencing offset: -0.02 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	118.0 ppm	external	indirect	1.0
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	42.6 ppm	external	indirect	1.0

Referencing offset: 0.36 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 2M9K, Strand ID: A, B Detail

Release date

2014-10-12

Citation

Homodimerization of RBPMS2 through a new RRM-interaction motif is necessary to control smooth muscle plasticity
Sagnol, S., Yang, Y., Bessin, Y., Allemand, F., Hapkova, I., Notarnicola, C., Guichou, J., Faure, S., Labesse, G., de Santa Barbara, P.
Nucleic Acids Res. (2014), 42, 10173-10184, PubMed 25064856 , DOI 10.1093/nar/gku692 , Abstract

Related entities 1. RBPMS2-Nter, : 1 : 260 entities Detail

Interaction partners 1. RBPMS2-Nter, : 214 interactors Detail

More details for 214 interactors identified by 13 citations

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DRX - 700 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 305 K, pH 6.3

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 15N]	0.4-0.8 mM
2	entity	[U-100% 13C; U-100% 15N]	0.7 mM
3	H2O	natural abundance	95 %
4	D2O	natural abundance	5 %

2 2D 1H-13C HSQC aliphatic

Instrument

Bruker DRX - 700 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 305 K, pH 6.3

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 15N]	0.4-0.8 mM
2	entity	[U-100% 13C; U-100% 15N]	0.7 mM
3	H2O	natural abundance	95 %
4	D2O	natural abundance	5 %

3 2D 1H-1H NOESY

Instrument

Bruker DRX - 700 MHz

Sample

State anisotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 305 K, pH 6.3

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 15N]	0.4-0.8 mM
2	entity	[U-100% 13C; U-100% 15N]	0.7 mM
3	H2O	natural abundance	95 %
4	D2O	natural abundance	5 %

4 3D HNCO

Instrument

Bruker DRX - 700 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 305 K, pH 6.3

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 15N]	0.4-0.8 mM
2	entity	[U-100% 13C; U-100% 15N]	0.7 mM
3	H2O	natural abundance	95 %
4	D2O	natural abundance	5 %

5 3D HNCA

Instrument

Bruker DRX - 700 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 305 K, pH 6.3

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 15N]	0.4-0.8 mM
2	entity	[U-100% 13C; U-100% 15N]	0.7 mM
3	H2O	natural abundance	95 %
4	D2O	natural abundance	5 %

6 3D CBCA(CO)NH

Instrument

Bruker DRX - 700 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 305 K, pH 6.3

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 15N]	0.4-0.8 mM
2	entity	[U-100% 13C; U-100% 15N]	0.7 mM
3	H2O	natural abundance	95 %
4	D2O	natural abundance	5 %

7 3D 1H-15N NOESY

Instrument

Bruker DRX - 700 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 305 K, pH 6.3

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 15N]	0.4-0.8 mM
2	entity	[U-100% 13C; U-100% 15N]	0.7 mM
3	H2O	natural abundance	95 %
4	D2O	natural abundance	5 %

8 3D 1H-13C NOESY aromatic

Instrument

Bruker DRX - 700 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 305 K, pH 6.3

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 15N]	0.4-0.8 mM
2	entity	[U-100% 13C; U-100% 15N]	0.7 mM
3	H2O	natural abundance	95 %
4	D2O	natural abundance	5 %

NMR combined restraints 6 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_19298_2m9k.nef
Input source #2: Coordindates	2m9k.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

---30--------40--------50--------60--------70--------80--------90-------100-------110--------
MEEEVRTLFVSGLPVDIKPRELYLLFRPFKGYEGSLIKLTARQPVGFVIFDSRAGAEAAKNALNGIRFDPENPQTLRLEFAKANTKMAKSKLE
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MEEEVRTLFVSGLPVDIKPRELYLLFRPFKGYEGSLIKLTARQPVGFVIFDSRAGAEAAKNALNGIRFDPENPQTLRLEFAKANTKMAKSKLE
--------10--------20--------30--------40--------50--------60--------70--------80--------90---

Entity_assembly_ID (NMR): B vs Auth_asym_ID (model): B

---30--------40--------50--------60--------70--------80--------90-------100-------110--------
MEEEVRTLFVSGLPVDIKPRELYLLFRPFKGYEGSLIKLTARQPVGFVIFDSRAGAEAAKNALNGIRFDPENPQTLRLEFAKANTKMAKSKLE
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MEEEVRTLFVSGLPVDIKPRELYLLFRPFKGYEGSLIKLTARQPVGFVIFDSRAGAEAAKNALNGIRFDPENPQTLRLEFAKANTKMAKSKLE
--------10--------20--------30--------40--------50--------60--------70--------80--------90---

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	93	0	0	100.0
B	B	93	0	0	100.0

Content subtype: combined_19298_2m9k.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	523		98.9 (chain: A, length: 93)
1	Distance restraints	DYANA/DIANA_distance_constraints_2	Warning	1359 (1)	noe	94.6 (chain: A, length: 93), 9.7 (chain: B, length: 93)
2	Distance restraints	DYANA/DIANA_distance_constraints_4	OK	48 (1)	noe	40.9 (chain: A, length: 93)
3	Distance restraints	DYANA/DIANA_distance_constraints_3	Warning	20 (1)	hbond	12.9 (chain: A, length: 93)
1	Dihedral angle restraints	DYANA/DIANA_dihedral_5	OK	308 (308)	.	91.4 (chain: A, length: 93), 91.4 (chain: B, length: 93)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 93, Sequence coverage: 98.9%
- Total number of assignments
  - ¹H chemical shifts: 182
  - ¹³C chemical shifts: 256
  - ¹⁵N chemical shifts: 85
- Completeness of assignments
  - Entity_assemble_ID: A
- Peptide bond of PRO
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	591	182	30.8
¹³C chemical shifts	440	256	58.2
¹⁵N chemical shifts	100	85	85.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	186	182	97.8
¹³C chemical shifts	186	177	95.2
¹⁵N chemical shifts	87	85	97.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	405	0	0.0
¹³C chemical shifts	254	79	31.1
¹⁵N chemical shifts	13	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	55	0	0.0
¹³C chemical shifts	55	9	16.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	43	0	0.0
¹³C chemical shifts	43	0	0.0

Distance restraints

Saveframe: DYANA/DIANA_distance_constraints_2
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 93, Sequence coverage: 94.6%
  - Entity_assembly_ID: B, Chain length: 93, Sequence coverage: 9.7%
- Total number of restraints: 1359
- Weight of restraints: 1.0 (1359)
- Potential type of restraints: upper-bound-parabolic (1359)
- Range of distance target values: 2.0, 8.4 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
  - Chain_ID: B
- Distance restraints on contact map
- Saveframe: DYANA/DIANA_distance_constraints_4
  - Status: OK
  - Experiment type: noe
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 93, Sequence coverage: 40.9%
  - Total number of restraints: 48
  - Weight of restraints: 1.0 (48)
  - Potential type of restraints: upper-bound-parabolic (48)
  - Range of distance target values: 2.0, 4.8 (Å)
  - Histogram of distance target values
  - Distance restraints per residue
    - Chain_ID: A
    - Chain_ID: B
  - Distance restraints on contact map
  - Saveframe: DYANA/DIANA_distance_constraints_3
    - Status: Warning
    - Experiment type: hbond
    - Sequence coverage of experimental data
      - Entity_assembly_ID: A, Chain length: 93, Sequence coverage: 12.9%
    - Total number of restraints: 20
    - Weight of restraints: 1.0 (20)
    - Potential type of restraints: upper-bound-parabolic (20)
    - Range of distance target values: 1.8, 2.7 (Å)
    - Histogram of distance target values
    - Distance restraints per residue
      - Chain_ID: A
      - Chain_ID: B
        None
    - Distance restraints on contact map

Dihedral angle restraints

Saveframe: DYANA/DIANA_dihedral_5
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 93, Sequence coverage: 91.4%
  - Entity_assembly_ID: B, Chain length: 93, Sequence coverage: 91.4%
- Total number of restraints: 308
- Total number of restraints per polymer type: 308
- Weight of restraints: 1.0 (308)
- Potential type of restraints: square-well-parabolic (308)
- Plot of Phi-Psi angle restraints
- Plot of Chi1-Chi2 angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A
  - Chain_ID: B

Keywords RBPMS2

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Found 1 Document (2.216 seconds)

BMRB: 19298

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. RBPMS2-Nter, : 1 : 260 entities Detail

Interaction partners 1. RBPMS2-Nter, : 214 interactors Detail

Experiments performed 8 experiments Detail

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NMR combined restraints 6 contents Detail