BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	79.6 % (591 of 742)	85.9 % (334 of 389)	70.4 % (200 of 284)	82.6 % (57 of 69)
Backbone	79.6 % (293 of 368)	92.9 % (117 of 126)	65.2 % (120 of 184)	96.6 % (56 of 58)
Sidechain	82.2 % (355 of 432)	82.5 % (217 of 263)	86.7 % (137 of 158)	9.1 % (1 of 11)
Aromatic	44.1 % (15 of 34)	88.2 % (15 of 17)	0.0 % (0 of 17)
Methyl	100.0 % (82 of 82)	100.0 % (41 of 41)	100.0 % (41 of 41)

1. FAAP24 HhH2

GSSEPSLLRT VQQIPGVGKV KAPLLLQKFP SIQQLSNASI GELEQVVGQA VAQQIHAFFT QPR

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	FAAP24_HhH2	[U-100% 13C; U-100% 15N]	0.5-0.8 mM
2	potassium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	beta-mercaptoethanol	natural abundance	1 mM
5	EDTA	natural abundance	0.5 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
8	FAAP24_HhH2	[U-100% 13C; U-100% 15N]	0.5-0.8 mM
9	potassium phosphate	natural abundance	50 mM
10	sodium chloride	natural abundance	50 mM
11	beta-mercaptoethanol	natural abundance	1 mM
12	EDTA	natural abundance	0.5 mM
13	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.43 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.43 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.28 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2M9N, Strand ID: A Detail

Release date

2013-09-15

Citation

Structure analysis of FAAP24 reveals single-stranded DNA-binding activity and domain functions in DNA damage response
Wang, Y., Han, X., Wu, F., Leung, J.W., Lowery, M.G., Do, H., Chen, J., Shi, C., Tian, C., Li, L., Gong, W.
Cell Res. (2013), 23, 1215-1228, PubMed 23999858 , DOI 10.1038/cr.2013.124 , Abstract

Related entities 1. (HhH)2 domain of human FAAP24, : 1 : 5 : 30 entities Detail

Interaction partners 1. (HhH)2 domain of human FAAP24, : 8 interactors Detail

More details for 8 interactors identified by 5 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DMX - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	FAAP24_HhH2	[U-100% 13C; U-100% 15N]	0.5-0.8 mM
2	potassium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	beta-mercaptoethanol	natural abundance	1 mM
5	EDTA	natural abundance	0.5 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

2 3D CBCA(CO)NH

Instrument

Bruker DMX - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	FAAP24_HhH2	[U-100% 13C; U-100% 15N]	0.5-0.8 mM
2	potassium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	beta-mercaptoethanol	natural abundance	1 mM
5	EDTA	natural abundance	0.5 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

3 3D HNCACB

Instrument

Bruker DMX - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	FAAP24_HhH2	[U-100% 13C; U-100% 15N]	0.5-0.8 mM
2	potassium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	beta-mercaptoethanol	natural abundance	1 mM
5	EDTA	natural abundance	0.5 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

4 3D HNCO

Instrument

Bruker DMX - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	FAAP24_HhH2	[U-100% 13C; U-100% 15N]	0.5-0.8 mM
2	potassium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	beta-mercaptoethanol	natural abundance	1 mM
5	EDTA	natural abundance	0.5 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

5 3D HNCA

Instrument

Bruker DMX - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	FAAP24_HhH2	[U-100% 13C; U-100% 15N]	0.5-0.8 mM
2	potassium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	beta-mercaptoethanol	natural abundance	1 mM
5	EDTA	natural abundance	0.5 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

6 3D HN(CO)CA

Instrument

Bruker DMX - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	FAAP24_HhH2	[U-100% 13C; U-100% 15N]	0.5-0.8 mM
2	potassium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	beta-mercaptoethanol	natural abundance	1 mM
5	EDTA	natural abundance	0.5 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

7 3D HBHA(CO)NH

Instrument

Bruker DMX - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	FAAP24_HhH2	[U-100% 13C; U-100% 15N]	0.5-0.8 mM
2	potassium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	beta-mercaptoethanol	natural abundance	1 mM
5	EDTA	natural abundance	0.5 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

8 3D H(CCO)NH

Instrument

Bruker DMX - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	FAAP24_HhH2	[U-100% 13C; U-100% 15N]	0.5-0.8 mM
2	potassium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	beta-mercaptoethanol	natural abundance	1 mM
5	EDTA	natural abundance	0.5 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

9 3D C(CO)NH

Instrument

Bruker DMX - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	FAAP24_HhH2	[U-100% 13C; U-100% 15N]	0.5-0.8 mM
2	potassium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	beta-mercaptoethanol	natural abundance	1 mM
5	EDTA	natural abundance	0.5 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

10 3D HCCH-TOCSY

Instrument

Bruker DMX - 850 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
8	FAAP24_HhH2	[U-100% 13C; U-100% 15N]	0.5-0.8 mM
9	potassium phosphate	natural abundance	50 mM
10	sodium chloride	natural abundance	50 mM
11	beta-mercaptoethanol	natural abundance	1 mM
12	EDTA	natural abundance	0.5 mM
13	D2O	natural abundance	100 %

11 3D HCCH-COSY

Instrument

Bruker DMX - 850 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
8	FAAP24_HhH2	[U-100% 13C; U-100% 15N]	0.5-0.8 mM
9	potassium phosphate	natural abundance	50 mM
10	sodium chloride	natural abundance	50 mM
11	beta-mercaptoethanol	natural abundance	1 mM
12	EDTA	natural abundance	0.5 mM
13	D2O	natural abundance	100 %

12 3D 1H-15N NOESY

Instrument

Bruker DMX - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	FAAP24_HhH2	[U-100% 13C; U-100% 15N]	0.5-0.8 mM
2	potassium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	beta-mercaptoethanol	natural abundance	1 mM
5	EDTA	natural abundance	0.5 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

13 3D 1H-13C NOESY

Instrument

Bruker DMX - 850 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
8	FAAP24_HhH2	[U-100% 13C; U-100% 15N]	0.5-0.8 mM
9	potassium phosphate	natural abundance	50 mM
10	sodium chloride	natural abundance	50 mM
11	beta-mercaptoethanol	natural abundance	1 mM
12	EDTA	natural abundance	0.5 mM
13	D2O	natural abundance	100 %

NMR combined restraints 4 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_19303_2m9n.nef
Input source #2: Coordindates	2m9n.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

-----160-------170-------180-------190-------200-------210-----
GSSEPSLLRTVQQIPGVGKVKAPLLLQKFPSIQQLSNASIGELEQVVGQAVAQQIHAFFTQPR
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
GSSEPSLLRTVQQIPGVGKVKAPLLLQKFPSIQQLSNASIGELEQVVGQAVAQQIHAFFTQPR
--------10--------20--------30--------40--------50--------60---

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	63	0	0	100.0

Content subtype: combined_19303_2m9n.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	585		96.8 (chain: A, length: 63)
1	Distance restraints	CNS/XPLOR_distance_constraints_3	OK	1103 (1)	noe	93.7 (chain: A, length: 63)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_2	OK	108 (108)	.	95.2 (chain: A, length: 63)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 63, Sequence coverage: 96.8%
- Total number of assignments
  - ¹H chemical shifts: 333
  - ¹³C chemical shifts: 196
  - ¹⁵N chemical shifts: 56
- Completeness of assignments
  - Entity_assemble_ID: A
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	389	333	85.6
¹³C chemical shifts	284	196	69.0
¹⁵N chemical shifts	71	56	78.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	126	119	94.4
¹³C chemical shifts	126	61	48.4
¹⁵N chemical shifts	58	56	96.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	263	214	81.4
¹³C chemical shifts	158	135	85.4
¹⁵N chemical shifts	13	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	41	41	100.0
¹³C chemical shifts	41	41	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	17	15	88.2
¹³C chemical shifts	17	0	0.0

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_3
- Status: OK
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 63, Sequence coverage: 93.7%
- Total number of restraints: 1103
- Weight of restraints: 1.0 (1103)
- Potential type of restraints: square-well-parabolic (1103)
- Range of distance target values: 2.4, 3.9 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_2
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 63, Sequence coverage: 95.2%
- Total number of restraints: 108
- Total number of restraints per polymer type: 108
- Weight of restraints: 1.0 (108)
- Potential type of restraints: square-well-parabolic (108)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A

Keywords FAAP24, Fanconi anemia, (HhH)2 domain, Solution NMR

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Found 1 Document (0.825 seconds)

BMRB: 19303

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. (HhH)2 domain of human FAAP24, : 1 : 5 : 30 entities Detail

Interaction partners 1. (HhH)2 domain of human FAAP24, : 8 interactors Detail

Experiments performed 13 experiments Detail

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NMR combined restraints 4 contents Detail