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BMRB: 19311

2M9V

Chemical shifts and structural restraints for Saccharomyces cerevisiae Est3 protein

Authors

Rao, T., Armstrong, G.S., Wuttke, D.S.

Assembly

Saccharomyces cerevisiae Est3 protein

Entity

1. Saccharomyces cerevisiae Est3 protein (polymer, Thiol state: all free), 170 monomers, 19277.67 Da Detail

STDSVFLQPW IKALIEDNSE HDQYHPSGHV IPSLTKQDLA LPHMSPTILT NPCHFAKITK FYNVCDYKVY ASIRDSSHQI LVEFSQECVS NFERTHNCRI TSETTNCLMI IGDADLVYVT NSRAMSHFKI SLSNISSKEI VPVLNVNQAT IFDIDQVGSL STFPFVYKYL

Formula weight

19277.67 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

Molecular fragment replacement

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.6 %, Completeness: 51.6 %, Completeness (bb): 79.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	51.6 % (1030 of 1996)	28.7 % (294 of 1025)	73.7 % (583 of 791)	85.0 % (153 of 180)
Backbone	79.4 % (797 of 1004)	50.7 % (170 of 335)	93.7 % (475 of 507)	93.8 % (152 of 162)
Sidechain	34.2 % (396 of 1159)	18.0 % (124 of 690)	60.1 % (271 of 451)	5.6 % (1 of 18)
Aromatic	2.1 % (4 of 190)	2.1 % (2 of 95)	2.1 % (2 of 94)	0.0 % (0 of 1)
Methyl	72.3 % (146 of 202)	69.3 % (70 of 101)	75.2 % (76 of 101)

1. entity

STDSVFLQPW IKALIEDNSE HDQYHPSGHV IPSLTKQDLA LPHMSPTILT NPCHFAKITK FYNVCDYKVY ASIRDSSHQI LVEFSQECVS NFERTHNCRI TSETTNCLMI IGDADLVYVT NSRAMSHFKI SLSNISSKEI VPVLNVNQAT IFDIDQVGSL STFPFVYKYL

Show sample condition

Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 7.1, Details Protein was 2H,15N,13C labeled. Sample concentration was 280 uM.

#	Name	Isotope labeling	Concentration
1	Est3 protein	[U-13C; U-15N; U-2H]	280 uM
2	Bis tris propane	natural abundance	50 mM
3	MOPS	natural abundance	150 mM
4	sodium sulfate	natural abundance	50 mM
5	arginine	natural abundance	100 mM
6	glutamate	natural abundance	100 mM
7	NDSB-195	natural abundance	100 mM
8	DTT	natural abundance	2 mM
9	D2O	natural abundance	7 %
10	H2O	natural abundance	93 %
11	TSP	natural abundance	0.15 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.34 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.34 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.41 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2M9V, Strand ID: A Detail

Release date

2013-12-15

Citation

Structure of Est3 reveals a bimodal surface with differential roles in telomere replication
Rao, T., Lubin, J.W., Armstrong, G.S., Tucey, T.M., Lundblad, V., Wuttke, D.S.
Proc. Natl. Acad. Sci. U. S. A. (2014), 111, 214-218, PubMed 24344315 , DOI 10.1073/pnas.1316453111 , Abstract

Related entities 1. Saccharomyces cerevisiae Est3 protein, : 1 : 2 : 3 entities Detail

Interaction partners 1. Saccharomyces cerevisiae Est3 protein, : 3 interactors Detail

More details for 3 interactors identified by 2 citations

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC TROSY