BMRBj - BMREntryMaster

Found 1 Document (0.789 seconds)

BMRB: 19319

2MA2

Solution structure of RasGRP2 EF hands bound to calcium

Authors

Kuriyan, J., Iwig, J., Vercoulen, Y., Das, R., Barros, T., Limnander, A., Che, Y., Pelton, J., Wemmer, D., Roose, J.

Assembly

RasGRP2 EF hands bound to calcium

Entity

1. RasGRP2 (polymer, Thiol state: all free), 81 monomers, 9206.176 Da Detail

KLDQALVVEH IEKMVESVFR NFDVDGDGHI SQEEFQIIRG NFPYLSAFGD LDQNQDGCIS REEMVSYFLR SSSVLGGRMG F

2. CALCIUM ION (non-polymer), 40.078 Da

Total weight

9246.254 Da

Max. entity weight

9206.176 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.8 %, Completeness: 76.4 %, Completeness (bb): 96.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	76.4 % (720 of 943)	69.0 % (338 of 490)	83.3 % (304 of 365)	88.6 % (78 of 88)
Backbone	96.7 % (468 of 484)	96.4 % (163 of 169)	97.0 % (228 of 235)	96.2 % (77 of 80)
Sidechain	60.9 % (324 of 532)	54.5 % (175 of 321)	72.9 % (148 of 203)	12.5 % (1 of 8)
Aromatic	5.3 % (5 of 94)	10.6 % (5 of 47)	0.0 % (0 of 47)
Methyl	89.5 % (68 of 76)	81.6 % (31 of 38)	97.4 % (37 of 38)

1. RasGRP2

KLDQALVVEH IEKMVESVFR NFDVDGDGHI SQEEFQIIRG NFPYLSAFGD LDQNQDGCIS REEMVSYFLR SSSVLGGRMG F

Show sample condition

Sample #1

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 304 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	RasGRP2	[U-99% 13C; U-99% 15N]	500 uM
2	HEPES	natural abundance	25 mM
3	sodium chloride	natural abundance	100 mM
4	TCEP	natural abundance	1 mM
5	D2O	natural abundance	7 %
6	H2O	natural abundance	93 %

Sample #2

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 304 K, pH 7.0

#	Name	Isotope labeling	Concentration
7	RasGRP2	[U-99% 13C; U-99% 15N]	500 uM
8	HEPES	natural abundance	25 mM
9	sodium chloride	natural abundance	100 mM
10	TCEP	natural abundance	1 mM
11	D2O	natural abundance	7 %
12	H2O	natural abundance	93 %
13	Pf1 phage	natural abundance	17 mg/mL

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.66 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2MA2, Strand ID: A Detail

Release date

2013-08-25

Citation

Structural analysis of autoinhibition in the Ras-specific exchange factor RasGRP1
Iwig, J., Vercoulen, Y., Das, R., Barros, T., Limnander, A., Che, Y., Pelton, J., Wemmer, D., Roose, J., Kuriyan, J.
Elife (2013), 2, e00813-e00813, PubMed 23908768 , DOI 10.7554/eLife.00813 , Abstract

Related entities 1. RasGRP2, : 1 : 2 : 1 : 81 entities Detail

Interaction partners 1. RasGRP2, : 3 interactors Detail

More details for 3 interactors identified by 2 citations

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 304 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	RasGRP2	[U-99% 13C; U-99% 15N]	500 uM
2	HEPES	natural abundance	25 mM
3	sodium chloride	natural abundance	100 mM
4	TCEP	natural abundance	1 mM
5	D2O	natural abundance	7 %
6	H2O	natural abundance	93 %

2 2D 1H-13C HSQC aromatic

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 304 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	RasGRP2	[U-99% 13C; U-99% 15N]	500 uM
2	HEPES	natural abundance	25 mM
3	sodium chloride	natural abundance	100 mM
4	TCEP	natural abundance	1 mM
5	D2O	natural abundance	7 %
6	H2O	natural abundance	93 %

3 3D CBCA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 304 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	RasGRP2	[U-99% 13C; U-99% 15N]	500 uM
2	HEPES	natural abundance	25 mM
3	sodium chloride	natural abundance	100 mM
4	TCEP	natural abundance	1 mM
5	D2O	natural abundance	7 %
6	H2O	natural abundance	93 %

4 3D C(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 304 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	RasGRP2	[U-99% 13C; U-99% 15N]	500 uM
2	HEPES	natural abundance	25 mM
3	sodium chloride	natural abundance	100 mM
4	TCEP	natural abundance	1 mM
5	D2O	natural abundance	7 %
6	H2O	natural abundance	93 %

5 3D HNCO

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 304 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	RasGRP2	[U-99% 13C; U-99% 15N]	500 uM
2	HEPES	natural abundance	25 mM
3	sodium chloride	natural abundance	100 mM
4	TCEP	natural abundance	1 mM
5	D2O	natural abundance	7 %
6	H2O	natural abundance	93 %

6 3D HNCACB

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 304 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	RasGRP2	[U-99% 13C; U-99% 15N]	500 uM
2	HEPES	natural abundance	25 mM
3	sodium chloride	natural abundance	100 mM
4	TCEP	natural abundance	1 mM
5	D2O	natural abundance	7 %
6	H2O	natural abundance	93 %

7 3D HNHA

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 304 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	RasGRP2	[U-99% 13C; U-99% 15N]	500 uM
2	HEPES	natural abundance	25 mM
3	sodium chloride	natural abundance	100 mM
4	TCEP	natural abundance	1 mM
5	D2O	natural abundance	7 %
6	H2O	natural abundance	93 %

8 2D 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 304 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	RasGRP2	[U-99% 13C; U-99% 15N]	500 uM
2	HEPES	natural abundance	25 mM
3	sodium chloride	natural abundance	100 mM
4	TCEP	natural abundance	1 mM
5	D2O	natural abundance	7 %
6	H2O	natural abundance	93 %

9 3D H(CCO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 304 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	RasGRP2	[U-99% 13C; U-99% 15N]	500 uM
2	HEPES	natural abundance	25 mM
3	sodium chloride	natural abundance	100 mM
4	TCEP	natural abundance	1 mM
5	D2O	natural abundance	7 %
6	H2O	natural abundance	93 %

10 2D 1H-15N HSQC IPAP

Instrument

Bruker Avance - 800 MHz

Sample

State anisotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 304 K, pH 7.0

#	Name	Isotope labeling	Concentration
7	RasGRP2	[U-99% 13C; U-99% 15N]	500 uM
8	HEPES	natural abundance	25 mM
9	sodium chloride	natural abundance	100 mM
10	TCEP	natural abundance	1 mM
11	D2O	natural abundance	7 %
12	H2O	natural abundance	93 %
13	Pf1 phage	natural abundance	17 mg/mL

11 2D HN(CO)CA IPAP

Instrument

Bruker Avance - 800 MHz

Sample

State anisotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 304 K, pH 7.0

#	Name	Isotope labeling	Concentration
7	RasGRP2	[U-99% 13C; U-99% 15N]	500 uM
8	HEPES	natural abundance	25 mM
9	sodium chloride	natural abundance	100 mM
10	TCEP	natural abundance	1 mM
11	D2O	natural abundance	7 %
12	H2O	natural abundance	93 %
13	Pf1 phage	natural abundance	17 mg/mL

12 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 304 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	RasGRP2	[U-99% 13C; U-99% 15N]	500 uM
2	HEPES	natural abundance	25 mM
3	sodium chloride	natural abundance	100 mM
4	TCEP	natural abundance	1 mM
5	D2O	natural abundance	7 %
6	H2O	natural abundance	93 %

13 3D 1H-13C NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 304 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	RasGRP2	[U-99% 13C; U-99% 15N]	500 uM
2	HEPES	natural abundance	25 mM
3	sodium chloride	natural abundance	100 mM
4	TCEP	natural abundance	1 mM
5	D2O	natural abundance	7 %
6	H2O	natural abundance	93 %

14 3D 1H-15N HSQC NOE 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 304 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	RasGRP2	[U-99% 13C; U-99% 15N]	500 uM
2	HEPES	natural abundance	25 mM
3	sodium chloride	natural abundance	100 mM
4	TCEP	natural abundance	1 mM
5	D2O	natural abundance	7 %
6	H2O	natural abundance	93 %

nullKeywords EF hand, protein

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Found 1 Document (0.789 seconds)

BMRB: 19319

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. RasGRP2, : 1 : 2 : 1 : 81 entities Detail

Interaction partners 1. RasGRP2, : 3 interactors Detail

Experiments performed 14 experiments Detail

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