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BMRB: 19320

2MMY

1H, 13C and 15N NMR assignments of RNA recognition motif of human Taf15

Authors

Kashyap, M., Ganguly, A., Bhavesh, N.

Assembly

Taf15

Entity

1. Taf15 (polymer, Thiol state: not present), 97 monomers, 10604.77 Da Detail

GSHMSDNNTI FVQGLGEGVS TDQVGEFFKQ IGIIKTNKKT GKPMINLYTD KDTGKPKGEA TVSFDDPPSA KAAIDWFDGK EFHGNIIKVS FATRRPE

Formula weight

10604.77 Da

Source organism

Exptl. method

Refine. method

distance geometry, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.2 %, Completeness (bb): 98.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	95.2 % (1067 of 1121)	94.4 % (552 of 585)	95.4 % (415 of 435)	99.0 % (100 of 101)
Backbone	98.6 % (564 of 572)	97.5 % (195 of 200)	99.3 % (278 of 280)	98.9 % (91 of 92)
Sidechain	92.8 % (589 of 635)	92.7 % (357 of 385)	92.5 % (223 of 241)	100.0 % (9 of 9)
Aromatic	91.8 % (90 of 98)	91.8 % (45 of 49)	91.7 % (44 of 48)	100.0 % (1 of 1)
Methyl	97.7 % (84 of 86)	100.0 % (43 of 43)	95.3 % (41 of 43)

1. Taf15

GSHMSDNNTI FVQGLGEGVS TDQVGEFFKQ IGIIKTNKKT GKPMINLYTD KDTGKPKGEA TVSFDDPPSA KAAIDWFDGK EFHGNIIKVS FATRRPE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.2, Details phosphate buffer, pH=6.2

#	Name	Isotope labeling	Type	Concentration
1	Taf15	[U-100% 13C; U-100% 15N]		1 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

LACS Plot; CA

Referencing offset: -0.29 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: -0.29 ppm, Outliers: 1 Detail

LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 2 Detail

LACS Plot; CO

Referencing offset: -0.03 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MMY, Strand ID: A Detail

Release date

2015-03-22

Citation

1H, 13C and 15N NMR assignments of RNA recognition motif of human Taf15
Kashyap, M., Ganguly, A., Bhavesh, N.
Not known

Related entities 1. Taf15, : 1 : 5 : 248 entities Detail

Interaction partners 1. Taf15, : 56 interactors Detail

More details for 56 interactors identified by 18 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.2, Details phosphate buffer, pH=6.2

#	Name	Isotope labeling	Type	Concentration
1	Taf15	[U-100% 13C; U-100% 15N]		1 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

2 2D 1H-13C HSQC aliphatic

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.2, Details phosphate buffer, pH=6.2

#	Name	Isotope labeling	Type	Concentration
1	Taf15	[U-100% 13C; U-100% 15N]		1 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

3 2D 1H-13C HSQC aromatic

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.2, Details phosphate buffer, pH=6.2

#	Name	Isotope labeling	Type	Concentration
1	Taf15	[U-100% 13C; U-100% 15N]		1 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

4 3D CBCA(CO)NH

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.2, Details phosphate buffer, pH=6.2

#	Name	Isotope labeling	Type	Concentration
1	Taf15	[U-100% 13C; U-100% 15N]		1 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

5 3D HNCO

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.2, Details phosphate buffer, pH=6.2

#	Name	Isotope labeling	Type	Concentration
1	Taf15	[U-100% 13C; U-100% 15N]		1 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

6 3D HNCA

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.2, Details phosphate buffer, pH=6.2

#	Name	Isotope labeling	Type	Concentration
1	Taf15	[U-100% 13C; U-100% 15N]		1 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

7 3D HNCACB

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.2, Details phosphate buffer, pH=6.2

#	Name	Isotope labeling	Type	Concentration
1	Taf15	[U-100% 13C; U-100% 15N]		1 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

8 3D HN(CO)CA

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.2, Details phosphate buffer, pH=6.2

#	Name	Isotope labeling	Type	Concentration
1	Taf15	[U-100% 13C; U-100% 15N]		1 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

9 3D 1H-15N NOESY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.2, Details phosphate buffer, pH=6.2

#	Name	Isotope labeling	Type	Concentration
1	Taf15	[U-100% 13C; U-100% 15N]		1 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

10 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.2, Details phosphate buffer, pH=6.2

#	Name	Isotope labeling	Type	Concentration
1	Taf15	[U-100% 13C; U-100% 15N]		1 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

11 3D 1H-13C NOESY aromatic

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.2, Details phosphate buffer, pH=6.2

#	Name	Isotope labeling	Type	Concentration
1	Taf15	[U-100% 13C; U-100% 15N]		1 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

12 3D H(CCO)NH

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.2, Details phosphate buffer, pH=6.2

#	Name	Isotope labeling	Type	Concentration
1	Taf15	[U-100% 13C; U-100% 15N]		1 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

13 3D C(CO)NH

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.2, Details phosphate buffer, pH=6.2

#	Name	Isotope labeling	Type	Concentration
1	Taf15	[U-100% 13C; U-100% 15N]		1 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

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